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Research Article

Recruitment of a SAP18-HDAC1 Complex into HIV-1 Virions and Its Requirement for Viral Replication

  • Masha Sorin equal contributor,

    equal contributor Contributed equally to this work with: Masha Sorin, Jennifer Cano

    Affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America

    X
  • Jennifer Cano equal contributor,

    equal contributor Contributed equally to this work with: Masha Sorin, Jennifer Cano

    Affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America

    X
  • Supratik Das,

    Affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America

    X
  • Sheeba Mathew,

    Affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America

    X
  • Xuhong Wu,

    Affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America

    X
  • Kelvin P. Davies,

    Affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America

    Current address: Department of Urology, Albert Einstein College of Medicine, Bronx, New York, United States of America

    X
  • Xuanling Shi,

    Affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America

    Current address: HIV Research Centre, Tsinghua University, Beijing, People's Republic of China

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  • S.-W. Grace Cheng,

    Affiliation: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America

    Current address: Genome Sciences Centre, BC Cancer Research Centre, Vancouver, British Columbia, Canada

    X
  • David Ott,

    Affiliation: Basic Research Program, SAIC–Frederick Inc., National Cancer Institute–Frederick, Frederick, Maryland, United States of America

    X
  • Ganjam V. Kalpana mail

    kalpana@aecom.yu.edu

    Affiliations: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America, Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America

    X
  • Published: June 05, 2009
  • DOI: 10.1371/journal.ppat.1000463

Reader Comments (1)

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Possible explanation for the lack of interaction with IN

Posted by murachel on 25 Jan 2010 at 16:52 GMT

fragment of SAP18 (aa 45–153
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.1000463#article1.body1.sec2.sec1.p2

The NMR structure of SAP18 shows that it has a ubiquitin fold (McCallum et al., Biochemistry 2006). Based on the structure, this truncation of SAP18 lacks an entire B-strand, and might be unfolded. This would explain why the interaction with IN is not picked up in the two hybrid screen.

No competing interests declared.