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Research Article

Kinome-Wide RNAi Screen Implicates at Least 5 Host Hepatocyte Kinases in Plasmodium Sporozoite Infection

  • Miguel Prudêncio equal contributor,

    equal contributor Contributed equally to this work with: Miguel Prudêncio, Cristina D. Rodrigues

    Affiliations: Unidade de Malária, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal, Instituto Gulbenkian de Ciência, Oeiras, Portugal

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  • Cristina D. Rodrigues equal contributor,

    equal contributor Contributed equally to this work with: Miguel Prudêncio, Cristina D. Rodrigues

    Affiliations: Unidade de Malária, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal, Instituto Gulbenkian de Ciência, Oeiras, Portugal

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  • Michael Hannus,

    Affiliation: Cenix BioScience GmbH, Dresden, Germany

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  • Cécilie Martin,

    Affiliation: Cenix BioScience GmbH, Dresden, Germany

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  • Eliana Real,

    Affiliation: Unidade de Malária, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal

    X
  • Lígia A. Gonçalves,

    Affiliation: Instituto Gulbenkian de Ciência, Oeiras, Portugal

    X
  • Céline Carret,

    Affiliation: Unidade de Malária, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal

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  • Robert Dorkin,

    Affiliation: Alnylam Pharmaceuticals, Cambridge, Massachusetts, United States of America

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  • Ingo Röhl,

    Affiliation: Roche Kulmbach GmbH, Kulmbach, Germany

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  • Kerstin Jahn-Hoffmann,

    Affiliation: Roche Kulmbach GmbH, Kulmbach, Germany

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  • Adrian J. F. Luty,

    Affiliation: Department of Medical Microbiology, University Medical Centre, Nijmegen, The Netherlands

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  • Robert Sauerwein,

    Affiliation: Department of Medical Microbiology, University Medical Centre, Nijmegen, The Netherlands

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  • Christophe J. Echeverri,

    Affiliation: Cenix BioScience GmbH, Dresden, Germany

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  • Maria M. Mota mail

    mmota@fm.ul.pt

    Affiliations: Unidade de Malária, Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal, Instituto Gulbenkian de Ciência, Oeiras, Portugal

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  • Published: November 07, 2008
  • DOI: 10.1371/journal.ppat.1000201

Reader Comments (1)

Post a new comment on this article

Question about siRNA validation in this manuscript?

Posted by Rusman on 17 Jun 2009 at 22:03 GMT

We were discussing this paper on Scientist Solutions forums recently and the following question came up.

"Also, they knocked down 727 genes that are in the kinome. They bought these siRNAs from Ambion. Check out the cost!

This is an ambitious experiment to say the least. So Im wondering how each siRNA was validated?"

Just thought Id give the authors a chance to comment.

link to discussion below (sorry if this viewed as spam)

http://www.scientistsolut...

No competing interests declared.