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Research Article

Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages

  • Adam E. Bennett,

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

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  • Kedar Narayan,

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

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  • Dan Shi,

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

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  • Lisa M. Hartnell,

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

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  • Karine Gousset,

    Affiliation: HIV Drug Resistance Program, NCI, Frederick, Maryland, United States of America

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  • Haifeng He,

    Affiliation: FEI Company, Hillsboro, Oregon, United States of America

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  • Bradley C. Lowekamp,

    Affiliation: National Library of Medicine, NIH, Bethesda, Maryland, United States of America

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  • Terry S. Yoo,

    Affiliation: National Library of Medicine, NIH, Bethesda, Maryland, United States of America

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  • Donald Bliss,

    Affiliation: National Library of Medicine, NIH, Bethesda, Maryland, United States of America

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  • Eric O. Freed,

    Affiliation: HIV Drug Resistance Program, NCI, Frederick, Maryland, United States of America

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  • Sriram Subramaniam mail

    ss1@nih.gov

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

    X
  • Published: September 25, 2009
  • DOI: 10.1371/journal.ppat.1000591

Reader Comments (1)

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Why is Fetal Calf Serum needed?

Posted by Neuromancer on 30 Oct 2009 at 08:57 GMT

Monocyte-derived macrophages (MDM) from healthy donors were grown in RPMI-1640 media supplemented with 10% fetal calf serum.
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.1000591#article1.body1.sec2.sec4.p1

It seems that RPMI-1640 media would be sufficient. Would fetal calf serum not introduce foreign cells that would fundamentally alter the MDM's?

No competing interests declared.

RE: Why is Fetal Calf Serum needed?

sriram replied to Neuromancer on 04 Nov 2009 at 04:07 GMT

Commercially available FCS (Fetal Calf Serum) that we and other laboratories use for tissue culture does not contain blood cells. It is sterile-filtered and also heat-inactivated at 56 degrees before use. There is therefore no possibility of introducing foreign cells.

No competing interests declared.