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Research Article

Primate Lentiviral Vpx Commandeers DDB1 to Counteract a Macrophage Restriction

  • Natalia Sharova equal contributor,

    equal contributor Contributed equally to this work with: Natalia Sharova, Yuanfei Wu

    Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

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  • Yuanfei Wu equal contributor,

    equal contributor Contributed equally to this work with: Natalia Sharova, Yuanfei Wu

    Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

    X
  • Xiaonan Zhu,

    Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

    X
  • Ruzena Stranska,

    Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

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  • Rajnish Kaushik,

    Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

    X
  • Mark Sharkey,

    Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

    X
  • Mario Stevenson mail

    Mario.stevenson@umassmed.edu

    Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

    X
  • Published: May 02, 2008
  • DOI: 10.1371/journal.ppat.1000057

Reader Comments (2)

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Data inconsistency

Posted by Nina on 07 May 2008 at 09:18 GMT

In figure 5a, HIV-1 vpr-deficient vpx-supplied particles after 48 hours infect macrophages with about 2 copies per cell , whereas the same particles show only about 0.3 copies per cell in figure 6a.

What MOIs have been used in the experiments?


RE: Data inconsistency

lakecomo replied to Nina on 12 May 2008 at 21:57 GMT

Nina,
We use a standard MOI which aims to achieve one copy per cell. However, in infections of primary macrophages, one sees significant donor to donor variation in the permissivity of macrophage cultures to viral infection with the same inoculum. It is not possible to conduct multiple infections with macrophages from the same donor and, as such, variablity in the extent of infection between experiments is unavoidable.