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Research Article

Functional and Genetic Analysis of Coronavirus Replicase-Transcriptase Proteins

  • Stanley G Sawicki,

    Affiliation: Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio, United States of America

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  • Dorothea L Sawicki,

    Affiliation: Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio, United States of America

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  • Diane Younker,

    Affiliation: Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio, United States of America

    ¤a Current address: CheCS-Environmental Health Systems, Houston, Texas, United States of America

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  • Yvonne Meyer,

    Affiliation: Institute of Virology, University of Würzburg, Würzburg, Germany

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  • Volker Thiel,

    Affiliation: Institute of Virology, University of Würzburg, Würzburg, Germany

    ¤b Current address: Research Department, Cantonal Hospital, St. Gallen, Switzerland

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  • Helen Stokes,

    Affiliation: Department of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom

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  • Stuart G Siddell mail

    To whom correspondence should be addressed. E-mail: stuart.siddell@bristol.ac.uk

    Affiliation: Department of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom

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  • Published: December 09, 2005
  • DOI: 10.1371/journal.ppat.0010039

Reader Comments (1)

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Alb"ts"6 "ts" mutation

Posted by Siddell on 12 Sep 2012 at 16:16 GMT

Alb ts6, A9494→ C
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.0010039#article1.body1.sec2.sec3.p1

Sparks et al. (J. Virol. 82:599, 2008) identified a V148A substitution in nsp5 as the mutation responsible for the temperature-sensitive ("ts") phenotype of Alb"ts"6. This conclusion is consistent with the sequencing data we obtained. Our attribution of the "ts" phenotype of Alb"ts"6 to a mutation in nsp4 (N258T) was based upon a single revertant isolate (T258N). We also found the V148A mutation in Alb"ts"6 but in our revertant this mutation was retained and combined with a second-site reversion (H134Y) in nsp5. Thus, we wish to propose that cistron 1 is redefined as extending from nsp5 to nsp10.

No competing interests declared.