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Research Article

Immune Clearance of Attenuated Rabies Virus Results in Neuronal Survival with Altered Gene Expression

  • Emily A. Gomme,

    Affiliation: Department of Microbiology and Immunology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America

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  • Christoph Wirblich,

    Affiliation: Department of Microbiology and Immunology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America

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  • Sankar Addya,

    Affiliation: Kimmel Cancer Center, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America

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  • Glenn F. Rall,

    Affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States of America

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  • Matthias J. Schnell mail

    Matthias.Schnell@jefferson.edu

    Affiliations: Department of Microbiology and Immunology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America, Kimmel Cancer Center, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America, Jefferson Vaccine Center, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America

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  • Published: October 11, 2012
  • DOI: 10.1371/journal.ppat.1002971

Reader Comments (1)

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Mice

Posted by se108 on 16 Oct 2012 at 11:50 GMT

If I have got this correct the study has compared the transcriptional profiles of eGFP neurones surviving Rabies virus infection to a heterogenous population of tdTomato expressing uninfected brain cells. Under these circumstances it would be expected to see differences in transcription profiles. Is it possible to confirm dysregulation of genes by comparing the same subtype of infected versus uninfected neurones?

No competing interests declared.

RE: Mice

matthiasschnell replied to se108 on 16 Oct 2012 at 14:19 GMT

Whereas transcription differences in a heterogenous populations of neurons are not unexpected we discovered differences for the function of neurons in general. However, this is certainly only a first step and laser capture microscopy will give use more information because we will be able analyze similar neurons (infected/uninfected) from the same area.

No competing interests declared.