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Research Article

Cryoelectron Tomography of HIV-1 Envelope Spikes: Further Evidence for Tripod-Like Legs

  • Ping Zhu,

    Affiliations: Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America, Center for Structural and Molecular Biology and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China

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  • Hanspeter Winkler,

    Affiliation: Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America

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  • Elena Chertova,

    Affiliation: AIDS Vaccine Program, SAIC Frederick, National Cancer Institute at Frederick, Frederick, Maryland, United States of America

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  • Kenneth A. Taylor,

    Affiliation: Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America

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  • Kenneth H. Roux mail

    roux@bio.fsu.edu

    Affiliation: Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America

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  • Published: November 14, 2008
  • DOI: 10.1371/journal.ppat.1000203

Reader Comments (5)

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More details about experimental conditions needed

Posted by mborgnia on 16 Nov 2008 at 00:00 GMT

The pellets were resuspended in 10 µl of PBS of which 3.5 µl was placed on a 300 mesh R2/1 Quantifoil grid (Quantifoil, Jena, Germany) for 1 min. Excess virus and buffer was blotted with filter paper.
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.1000203#article1.body1.sec2.sec2.p1

Temperature?
Relative Humidity?
Blotting time?
Ice thickness?