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Research Article

HDP—A Novel Heme Detoxification Protein from the Malaria Parasite

  • Dewal Jani equal contributor,

    equal contributor Contributed equally to this work with: Dewal Jani, Rana Nagarkatti

    Affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, Virginia, United States of America

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  • Rana Nagarkatti equal contributor,

    equal contributor Contributed equally to this work with: Dewal Jani, Rana Nagarkatti

    Affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, Virginia, United States of America

    X
  • Wandy Beatty,

    Affiliation: Washington University School of Medicine, St. Louis, Missouri, United States of America

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  • Ross Angel,

    Affiliation: Department of Geosciences, Virginia Tech, Blacksburg, Virginia, United States of America

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  • Carla Slebodnick,

    Affiliation: Department of Chemistry, Virginia Tech, Blacksburg, Virginia, United States of America

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  • John Andersen,

    Affiliation: Laboratory of Malaria and Vector Research, National Institutes of Health, Rockville, Maryland, United States of America

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  • Sanjai Kumar,

    Affiliation: Food and Drug Administration, Bethesda, Maryland, United States of America

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  • Dharmendar Rathore mail

    Rathore@vbi.vt.edu

    Affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, Virginia, United States of America

    X
  • Published: April 25, 2008
  • DOI: 10.1371/journal.ppat.1000053

Reader Comments (2)

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Publisher's Note: Statements about use of stop codons is incorrect

Posted by MWMather on 26 Sep 2008 at 18:44 GMT

primers with in frame stop codons. This fragment was cloned into pHD22Y using the Kpn I restriction enzyme site to yield pHDPKO. The stop codons in the forward and reverse primers would prevent expression of the truncated HDP in the event of site specific recombination
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.1000053#article1.body1.sec4.sec6.p1

Due to incorrect interpretation of homologous recombination (see comment in discussions), authors place a stop codon preceding the truncated HDP gene in Figure 5A. There will not be a stop codon at this position following homologous recombination, hence the truncated gene will likely be expressed. The diagrams and annotations showing the anticipated outcomes of recombination in Fig 5A and B and Fig 6A and B are incorrect and need to be re-drawn.