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Research Article

Activation of Akt Signaling Reduces the Prevalence and Intensity of Malaria Parasite Infection and Lifespan in Anopheles stephensi Mosquitoes

  • Vanessa Corby-Harris equal contributor,

    equal contributor Contributed equally to this work with: Vanessa Corby-Harris, Anna Drexler

    Affiliation: Department of Entomology, University of Arizona, Tucson, Arizona, United States of America

    X
  • Anna Drexler equal contributor,

    equal contributor Contributed equally to this work with: Vanessa Corby-Harris, Anna Drexler

    Affiliation: Medical Microbiology and Immunology, University of California – Davis, Davis, California, United States of America

    X
  • Laurel Watkins de Jong,

    Affiliation: Department of Entomology, University of Arizona, Tucson, Arizona, United States of America

    X
  • Yevgeniya Antonova,

    Affiliation: Department of Entomology, University of Arizona, Tucson, Arizona, United States of America

    X
  • Nazzy Pakpour,

    Affiliation: Medical Microbiology and Immunology, University of California – Davis, Davis, California, United States of America

    X
  • Rolf Ziegler,

    Affiliation: Department of Entomology, University of Arizona, Tucson, Arizona, United States of America

    X
  • Frank Ramberg,

    Affiliation: Department of Entomology, University of Arizona, Tucson, Arizona, United States of America

    X
  • Edwin E. Lewis,

    Affiliations: Department of Entomology, University of California – Davis, Davis, California, United States of America, Department of Nematology, University of California – Davis, Davis, California, United States of America

    X
  • Jessica M. Brown,

    Affiliation: Department of Entomology, University of Arizona, Tucson, Arizona, United States of America

    X
  • Shirley Luckhart,

    Affiliation: Medical Microbiology and Immunology, University of California – Davis, Davis, California, United States of America

    X
  • Michael A. Riehle mail

    mriehle@ag.arizona.edu

    Affiliation: Department of Entomology, University of Arizona, Tucson, Arizona, United States of America

    X
  • Published: July 15, 2010
  • DOI: 10.1371/journal.ppat.1001003

Reader Comments (1)

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Membrane localization of the transgene

Posted by anton_scott_gousti on 18 Jul 2010 at 13:01 GMT

The legend to Fig. 3 is "Membrane localization of the transgene." I believe they mean "Membrane localization of the protein product of the transgene" not the transgene (DNA). Even so, I have a question for the authors. In mammals, Akt is found associated with both the plasma membrane and the nucleus. At its plasma membrane site, Akt co-operates with Pdk1 which can sense plasma-membrane associated PIP3 (a phosphorylated inositol) driven by PI-3-kinase activation. Nuclear Akt activation is driven by a different process. Does your transgenic protein product blunt nuclear Akt as well as PM-associated Akt?

No competing interests declared.

RE: Membrane localization of the transgene

mriehle replied to anton_scott_gousti on 20 Jul 2010 at 00:01 GMT

You are correct that in figure 3 it is the protein product of the transgene and not the transgene itself. The Akt transgene is expressed in an active form and upregulates downstream insulin signaling molecules. We did not observe any of our modified Akt in the nucleus and therefore we do not believe that nuclear Akt activity would be affected. However, this was not tested.

No competing interests declared.