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Research Article

A Point Mutation in a Herpesvirus Polymerase Determines Neuropathogenicity

  • Laura B Goodman,

    Affiliation: Department of Microbiology and Immunology, Cornell University, Ithaca, New York, United States of America

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  • Arianna Loregian,

    Affiliation: Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, Italy

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  • Gillian A Perkins,

    Affiliation: Department of Clinical Sciences, Cornell University, Ithaca, New York, United States of America

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  • Josie Nugent,

    Affiliation: Centre for Preventive Medicine, Animal Health Trust, Newmarket, United Kingdom

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  • Elizabeth L Buckles,

    Affiliation: Department of Biomedical Sciences, Cornell University, Ithaca, New York, United States of America

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  • Beatrice Mercorelli,

    Affiliation: Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, Italy

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  • Julia H Kydd,

    Affiliation: Centre for Preventive Medicine, Animal Health Trust, Newmarket, United Kingdom

    ¤a Current address: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, United Kingdom

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  • Giorgio Palù,

    Affiliation: Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, Italy

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  • Ken C Smith,

    Affiliation: Centre for Preventive Medicine, Animal Health Trust, Newmarket, United Kingdom

    ¤b Current address: Royal Veterinary College, Department of Pathology and Infectious Diseases, London, United Kingdom

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  • Nikolaus Osterrieder mail,

    To whom correspondence should be addressed. E-mail: no34@cornell.edu

    Affiliation: Department of Microbiology and Immunology, Cornell University, Ithaca, New York, United States of America

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  • Nicholas Davis-Poynter

    Affiliation: Centre for Preventive Medicine, Animal Health Trust, Newmarket, United Kingdom

    ¤c Current address: Herpesvirus Molecular Pathogenesis Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital Clinical Medical Virology Centre, University of Queensland, Queensland, Australia

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  • Published: November 09, 2007
  • DOI: 10.1371/journal.ppat.0030160

About the Authors

Laura B Goodman, Nikolaus Osterrieder
Department of Microbiology and Immunology, Cornell University, Ithaca, New York, United States of America
Arianna Loregian, Beatrice Mercorelli, Giorgio Palù
Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, Italy
Gillian A Perkins
Department of Clinical Sciences, Cornell University, Ithaca, New York, United States of America
Josie Nugent, Julia H Kydd, Ken C Smith, Nicholas Davis-Poynter
Centre for Preventive Medicine, Animal Health Trust, Newmarket, United Kingdom
Elizabeth L Buckles
Department of Biomedical Sciences, Cornell University, Ithaca, New York, United States of America

Corresponding Author

Email: no34@cornell.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

LBG performed the majority of the experiments, analyzed the data, and wrote the manuscript. AL, BM, and GP designed and performed the polymerase activity assays. ELB, KCS, and GAP performed postmortem examinations of animals. GAP, JN, and JHK participated in the animal studies, and GAP assisted with qPCR analyses. LBG, AL, GAP, JN, ELB, BM, JHK, GP, KCS, NO, and NDP revised the manuscript. NO and NDP share senior authorship.