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Research Article

Elite Suppressor–Derived HIV-1 Envelope Glycoproteins Exhibit Reduced Entry Efficiency and Kinetics

  • Kara G. Lassen equal contributor,

    equal contributor Contributed equally to this work with: Kara G. Lassen, Michael A. Lobritz

    Affiliation: Department of Molecular Biology and Microbiology, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America

    Current address: Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, California, United States of America

    X
  • Michael A. Lobritz equal contributor,

    equal contributor Contributed equally to this work with: Kara G. Lassen, Michael A. Lobritz

    Affiliations: Department of Molecular Biology and Microbiology, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America, Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America

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  • Justin R. Bailey,

    Affiliation: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

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  • Samantha Johnston,

    Affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America

    X
  • Sandra Nguyen,

    Affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America

    X
  • Benhur Lee,

    Affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America

    X
  • Tom Chou,

    Affiliation: Departments of Biomathematics and Mathematics, University of California Los Angeles, Los Angeles, California, United States of America

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  • Robert F. Siliciano,

    Affiliations: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

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  • Martin Markowitz,

    Affiliation: Aaron Diamond AIDS Research Center, Rockefeller University, New York, New York, United States of America

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  • Eric J. Arts mail

    eja3@case.edu

    Affiliation: Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America

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  • Published: April 10, 2009
  • DOI: 10.1371/journal.ppat.1000377

About the Authors

Kara G. Lassen, Michael A. Lobritz
Department of Molecular Biology and Microbiology, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America
Michael A. Lobritz, Eric J. Arts
Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America
Justin R. Bailey, Robert F. Siliciano
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
Samantha Johnston, Sandra Nguyen, Benhur Lee
Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America
Tom Chou
Departments of Biomathematics and Mathematics, University of California Los Angeles, Los Angeles, California, United States of America
Robert F. Siliciano
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
Martin Markowitz
Aaron Diamond AIDS Research Center, Rockefeller University, New York, New York, United States of America

Corresponding Author

Email: eja3@case.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: KGL MAL. Performed the experiments: KGL MAL. Analyzed the data: KGL MAL TC. Contributed reagents/materials/analysis tools: JRB SJ SN BL RFS MM EJA. Wrote the paper: KGL MAL. Provided reagents (envs), collected and cloned all of the HIV-1 envs from elite and acute patients, involved in experimental design and prep of the manuscript: JRB. Developed and tested the cell line and Affinofile system, assisted in experimental design and determination of the levels of CCR5 and CD4 in the cell system: SJ. Developed and tested the cell line and Affinofile system, assisted in experimental design and determination of the levels of CCR5 and CD4 in the cell system: SN. Developed and tested the cell line and Affinofile system, assisted in experimental design and determination of the levels of CCR5 and CD4 in the cell system, supervised activities, helped write manuscript and guide co-authors on the use of the Affinofile system: BL. Provided reagents (envs), supervised activities and reviewed the re-write of the manuscript: RFS. Identified the appropriate acutely infected patients, provided the plasma samples for this study, and was involved in providing clinical characteristics and in writing the manuscript: MM. Manuscript preparation, experimental design, oversight and guidance on all of the experiments and figure preparation: EJA.