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Research Article

High Throughput Functional Assays of the Variant Antigen PfEMP1 Reveal a Single Domain in the 3D7 Plasmodium falciparum Genome that Binds ICAM1 with High Affinity and Is Targeted by Naturally Acquired Neutralizing Antibodies

  • Andrew V. Oleinikov mail,

    andrew.oleinikov@sbri.org

    Affiliation: Seattle Biomedical Research Institute, Seattle, Washington, United States of America

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  • Emily Amos,

    Affiliation: Seattle Biomedical Research Institute, Seattle, Washington, United States of America

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  • Isaac Tyler Frye,

    Affiliation: Seattle Biomedical Research Institute, Seattle, Washington, United States of America

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  • Eddie Rossnagle,

    Affiliation: Seattle Biomedical Research Institute, Seattle, Washington, United States of America

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  • Theonest K. Mutabingwa,

    Affiliations: Seattle Biomedical Research Institute, Seattle, Washington, United States of America, National Institute for Medical Research, Dar es Salaam, Tanzania

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  • Michal Fried,

    Affiliations: Seattle Biomedical Research Institute, Seattle, Washington, United States of America, Department of Global Health, University of Washington, Seattle, Washington, United States of America

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  • Patrick E. Duffy

    Affiliations: Seattle Biomedical Research Institute, Seattle, Washington, United States of America, Department of Global Health, University of Washington, Seattle, Washington, United States of America

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  • Published: April 17, 2009
  • DOI: 10.1371/journal.ppat.1000386

About the Authors

Andrew V. Oleinikov, Emily Amos, Isaac Tyler Frye, Eddie Rossnagle, Theonest K. Mutabingwa, Michal Fried, Patrick E. Duffy
Seattle Biomedical Research Institute, Seattle, Washington, United States of America
Theonest K. Mutabingwa
National Institute for Medical Research, Dar es Salaam, Tanzania
Michal Fried, Patrick E. Duffy
Department of Global Health, University of Washington, Seattle, Washington, United States of America

Corresponding Author

Email: andrew.oleinikov@sbri.org

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: AVO PED. Performed the experiments: AVO EA ITF ER. Analyzed the data: AVO PED. Contributed reagents/materials/analysis tools: AVO TKM MF. Wrote the paper: AVO PED.