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Research Article

Comprehensive Identification of Salmonella enterica Serovar Typhimurium Genes Required for Infection of BALB/c Mice

  • Roy R. Chaudhuri equal contributor,

    equal contributor Contributed equally to this work with: Roy R. Chaudhuri, Sarah E. Peters

    Affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

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  • Sarah E. Peters equal contributor,

    equal contributor Contributed equally to this work with: Roy R. Chaudhuri, Sarah E. Peters

    Affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

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  • Stephen J. Pleasance,

    Affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

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  • Helen Northen,

    Affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

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  • Chrissie Willers,

    Affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

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  • Gavin K. Paterson,

    Affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

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  • Danielle B. Cone,

    Affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

    Current address: Health Protection Agency, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, United Kingdom

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  • Andrew G. Allen,

    Affiliation: Arrow Therapeutics Ltd., London, United Kingdom

    Current address: Pfizer Australia, Veterinary Medicine Research and Development, Parkville, Victoria, Australia

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  • Paul J. Owen,

    Affiliation: Arrow Therapeutics Ltd., London, United Kingdom

    Current address: Cancer Research Technology Development Laboratory, Wolfson Institute for Biomedical Research, London, United Kingdom

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  • Gil Shalom,

    Affiliation: Arrow Therapeutics Ltd., London, United Kingdom

    Current address: Division of Microbial Diseases, UCL Eastman Dental Institute, London, United Kingdom

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  • Dov J. Stekel,

    Affiliation: Centre for Systems Biology, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom

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  • Ian G. Charles ,

    Affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom

    These authors are joint last authors on this work.

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  • Duncan J. Maskell mail

    djm47@cam.ac.uk

    Affiliation: Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

    These authors are joint last authors on this work.

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  • Published: July 31, 2009
  • DOI: 10.1371/journal.ppat.1000529

About the Authors

Roy R. Chaudhuri, Sarah E. Peters, Stephen J. Pleasance, Helen Northen, Chrissie Willers, Gavin K. Paterson, Danielle B. Cone, Duncan J. Maskell
Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom
Andrew G. Allen, Paul J. Owen, Gil Shalom
Arrow Therapeutics Ltd., London, United Kingdom
Dov J. Stekel
Centre for Systems Biology, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
Ian G. Charles
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom

Corresponding Author

Email: djm47@cam.ac.uk

Competing Interests

DJM and IGC are named inventors on the patent “Transposon Mediated Differential Hybridisation”, International Patent Number WO2001/007651. DJM, IGC, AGA and PJO are named inventors on the patent “Transposon”, International Patent Number WO2003/074700.

Author Contributions

Conceived and designed the experiments: RRC SEP AGA PJO GS IGC DJM. Performed the experiments: SEP SJP HN CW GKP DBC AGA PJO GS. Analyzed the data: RRC. Wrote the paper: RRC SEP IGC DJM. Developed the microarray analysis methods: RRC. Developed the transposons: SEP AGA PJO IGC DJM. Performed the initial development of the TMDH method: SEP AGA PJO GS IGC DJM. Performed the mouse screens: SEP HN CW AGA. Performed the array experiments and verification of the locations of transposon insertions: SJP. Generated and tested the individual mutants: SEP HN CW GKP DBC. Contributed to development of microarray analysis methods: DJS. Invented the TMDH approach: IGC DJM.