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Research Article

Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages

  • Adam E. Bennett,

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

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  • Kedar Narayan,

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

    X
  • Dan Shi,

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

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  • Lisa M. Hartnell,

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

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  • Karine Gousset,

    Affiliation: HIV Drug Resistance Program, NCI, Frederick, Maryland, United States of America

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  • Haifeng He,

    Affiliation: FEI Company, Hillsboro, Oregon, United States of America

    X
  • Bradley C. Lowekamp,

    Affiliation: National Library of Medicine, NIH, Bethesda, Maryland, United States of America

    X
  • Terry S. Yoo,

    Affiliation: National Library of Medicine, NIH, Bethesda, Maryland, United States of America

    X
  • Donald Bliss,

    Affiliation: National Library of Medicine, NIH, Bethesda, Maryland, United States of America

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  • Eric O. Freed,

    Affiliation: HIV Drug Resistance Program, NCI, Frederick, Maryland, United States of America

    X
  • Sriram Subramaniam mail

    ss1@nih.gov

    Affiliation: Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America

    X
  • Published: September 25, 2009
  • DOI: 10.1371/journal.ppat.1000591

About the Authors

Adam E. Bennett, Kedar Narayan, Dan Shi, Lisa M. Hartnell, Sriram Subramaniam
Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America
Karine Gousset, Eric O. Freed
HIV Drug Resistance Program, NCI, Frederick, Maryland, United States of America
Haifeng He
FEI Company, Hillsboro, Oregon, United States of America
Bradley C. Lowekamp, Terry S. Yoo, Donald Bliss
National Library of Medicine, NIH, Bethesda, Maryland, United States of America

Corresponding Author

Email: ss1@nih.gov

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: AEB KN DS EOF SS. Performed the experiments: AEB KN DS LH HH SS. Analyzed the data: AEB KN DS LH BCL TSY DB SS. Contributed reagents/materials/analysis tools: KG. Wrote the paper: SS. Designed approach to effectively conduct the 3D tomo data collection, helped with sample preparation: HH.