Advertisement
Research Article

High Content Screening Identifies Decaprenyl-Phosphoribose 2′ Epimerase as a Target for Intracellular Antimycobacterial Inhibitors

  • Thierry Christophe,

    Affiliation: Screening Technologies and Pharmacology, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Mary Jackson,

    Affiliation: Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America

    X
  • Hee Kyoung Jeon,

    Affiliation: Screening Technologies and Pharmacology, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Denis Fenistein,

    Affiliation: Image Mining, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Monica Contreras-Dominguez,

    Affiliation: Biology of Intracellular Pathogens Inserm Avenir Group, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Jaeseung Kim,

    Affiliation: Medicinal Chemistry, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Auguste Genovesio,

    Affiliation: Image Mining, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Jean-Philippe Carralot,

    Affiliation: Biology of Intracellular Pathogens Inserm Avenir Group, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Fanny Ewann,

    Affiliation: Biology of Intracellular Pathogens Inserm Avenir Group, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Eun Hye Kim,

    Affiliation: Biology of Intracellular Pathogens Inserm Avenir Group, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Sae Yeon Lee,

    Affiliation: Medicinal Chemistry, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Sunhee Kang,

    Affiliation: Medicinal Chemistry, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Min Jung Seo,

    Affiliation: Medicinal Chemistry, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Eun Jung Park,

    Affiliation: Medicinal Chemistry, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Henrieta Škovierová,

    Affiliation: Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America

    X
  • Ha Pham,

    Affiliation: Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America

    X
  • Giovanna Riccardi,

    Affiliation: Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Pavia, Italy

    X
  • Ji Youn Nam,

    Affiliation: Screening Technologies and Pharmacology, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Laurent Marsollier,

    Affiliation: Groupe d'Etude des Interactions Hôte Pathogène, Université d'Angers, Angers, France

    X
  • Marie Kempf,

    Affiliation: Groupe d'Etude des Interactions Hôte Pathogène, Université d'Angers, Angers, France

    X
  • Marie-Laure Joly-Guillou,

    Affiliation: Groupe d'Etude des Interactions Hôte Pathogène, Université d'Angers, Angers, France

    X
  • Taegwon Oh,

    Affiliation: International Tuberculosis Research Center, Masan, Korea

    X
  • Won Kyung Shin,

    Affiliation: International Tuberculosis Research Center, Masan, Korea

    X
  • Zaesung No,

    Affiliation: Medicinal Chemistry, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Ulf Nehrbass,

    Affiliation: Screening Technologies and Pharmacology, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea

    X
  • Roland Brosch,

    Affiliation: Institut Pasteur, Integrated Mycobacterial Pathogenomics, Paris, France

    X
  • Stewart T. Cole,

    Affiliation: Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    X
  • Priscille Brodin mail

    pbrodin@ip-korea.org

    Affiliations: Biology of Intracellular Pathogens Inserm Avenir Group, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea, Institut Pasteur, Integrated Mycobacterial Pathogenomics, Paris, France

    X
  • Published: October 30, 2009
  • DOI: 10.1371/journal.ppat.1000645

About the Authors

Thierry Christophe, Hee Kyoung Jeon, Ji Youn Nam, Ulf Nehrbass
Screening Technologies and Pharmacology, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea
Mary Jackson, Henrieta Škovierová, Ha Pham
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
Denis Fenistein, Auguste Genovesio
Image Mining, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea
Monica Contreras-Dominguez, Jean-Philippe Carralot, Fanny Ewann, Eun Hye Kim, Priscille Brodin
Biology of Intracellular Pathogens Inserm Avenir Group, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea
Jaeseung Kim, Sae Yeon Lee, Sunhee Kang, Min Jung Seo, Eun Jung Park, Zaesung No
Medicinal Chemistry, Institut Pasteur Korea, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea
Giovanna Riccardi
Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Pavia, Italy
Laurent Marsollier, Marie Kempf, Marie-Laure Joly-Guillou
Groupe d'Etude des Interactions Hôte Pathogène, Université d'Angers, Angers, France
Taegwon Oh, Won Kyung Shin
International Tuberculosis Research Center, Masan, Korea
Roland Brosch, Priscille Brodin
Institut Pasteur, Integrated Mycobacterial Pathogenomics, Paris, France
Stewart T. Cole
Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

Corresponding Author

Email: pbrodin@ip-korea.org

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: TC MJ HKJ DF JK ZN PB. Performed the experiments: TC HKJ MCD JK FE EHK SYL SK MJS EJP HS HP JYN MK TO WKS PB. Analyzed the data: TC MJ HKJ JK FE JYN MK TO PB. Contributed reagents/materials/analysis tools: TC DF JK AG JPC GR LM MLJG WKS UN PB. Wrote the paper: TC MJ DF JK RB STC PB.