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Research Article

Caenorhabditis elegans N-glycan Core β-galactoside Confers Sensitivity towards Nematotoxic Fungal Galectin CGL2

  • Alex Butschi equal contributor,

    equal contributor Contributed equally to this work with: Alex Butschi, Alexander Titz, Martin A. Wälti

    Affiliation: Institute of Molecular Biology, University of Zürich, Zürich, Switzerland

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  • Alexander Titz equal contributor,

    equal contributor Contributed equally to this work with: Alex Butschi, Alexander Titz, Martin A. Wälti

    Affiliation: Institute of Microbiology, Swiss Federal Institute of Technology (ETH) Zürich, Zürich, Switzerland

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  • Martin A. Wälti equal contributor,

    equal contributor Contributed equally to this work with: Alex Butschi, Alexander Titz, Martin A. Wälti

    Affiliation: Institute of Microbiology, Swiss Federal Institute of Technology (ETH) Zürich, Zürich, Switzerland

    Current address: Institute of Zoology, University of Zürich, Zürich, Switzerland

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  • Vincent Olieric,

    Affiliation: Swiss Light Source (SLS), Paul-Scherrer-Institute (PSI), Villigen, Switzerland

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  • Katharina Paschinger,

    Affiliation: Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria

    X
  • Katharina Nöbauer,

    Affiliation: VetOMICS Core Facility for Proteomics & Metabolomics Studies, University of Veterinary Medicine, Vienna, Austria

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  • Xiaoqiang Guo,

    Affiliation: Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland

    Current address: Max Planck Institute of Colloids and Interfaces, Potsdam-Golm, Germany

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  • Peter H. Seeberger,

    Affiliation: Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland

    Current address: Max Planck Institute of Colloids and Interfaces, Potsdam-Golm, Germany

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  • Iain B. H. Wilson,

    Affiliation: Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria

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  • Markus Aebi,

    Affiliation: Institute of Microbiology, Swiss Federal Institute of Technology (ETH) Zürich, Zürich, Switzerland

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  • Michael O. Hengartner,

    Affiliation: Institute of Molecular Biology, University of Zürich, Zürich, Switzerland

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  • Markus Künzler mail

    markus.kuenzler@micro.biol.ethz.ch

    Affiliation: Institute of Microbiology, Swiss Federal Institute of Technology (ETH) Zürich, Zürich, Switzerland

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  • Published: January 08, 2010
  • DOI: 10.1371/journal.ppat.1000717

About the Authors

Alex Butschi, Michael O. Hengartner
Institute of Molecular Biology, University of Zürich, Zürich, Switzerland
Alexander Titz, Martin A. Wälti, Markus Aebi, Markus Künzler
Institute of Microbiology, Swiss Federal Institute of Technology (ETH) Zürich, Zürich, Switzerland
Vincent Olieric
Swiss Light Source (SLS), Paul-Scherrer-Institute (PSI), Villigen, Switzerland
Katharina Paschinger, Iain B. H. Wilson
Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria
Katharina Nöbauer
VetOMICS Core Facility for Proteomics & Metabolomics Studies, University of Veterinary Medicine, Vienna, Austria
Xiaoqiang Guo, Peter H. Seeberger
Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland

Corresponding Author

Email: markus.kuenzler@micro.biol.ethz.ch

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: IBHW MA MOH MK. Performed the experiments: AB AT MAW VO KP XG. Analyzed the data: AB AT MAW VO KP XG. Contributed reagents/materials/analysis tools: VO KN PHS IBHW MA MOH. Wrote the paper: MK. Performed the C. elegans toxicity assays, the Mos1 screen and the microscopy: AB. Performed the N-glycan analysis (with help of KP and KN) and the ITC measurements (with help of MAW): AT. Discovered the nematotoxicity of CGL2, purified CGL2 and produced the CGL2 crystals: MAW. Determined the X-ray structure: VO. Synthesized the trisaccharide: XG.