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Research Article

Direct Visualization by Cryo-EM of the Mycobacterial Capsular Layer: A Labile Structure Containing ESX-1-Secreted Proteins

  • Musa Sani equal contributor,

    equal contributor Contributed equally to this work with: Musa Sani, Edith N. G. Houben, Jeroen Geurtsen

    Affiliation: Division of Cell Biology-B6, Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital (NKI-AVL), Amsterdam, the Netherlands

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  • Edith N. G. Houben equal contributor,

    equal contributor Contributed equally to this work with: Musa Sani, Edith N. G. Houben, Jeroen Geurtsen

    Affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, the Netherlands

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  • Jeroen Geurtsen equal contributor,

    equal contributor Contributed equally to this work with: Musa Sani, Edith N. G. Houben, Jeroen Geurtsen

    Affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, the Netherlands

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  • Jason Pierson,

    Affiliation: Division of Cell Biology-B6, Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital (NKI-AVL), Amsterdam, the Netherlands

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  • Karin de Punder,

    Affiliation: Division of Cell Biology-B6, Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital (NKI-AVL), Amsterdam, the Netherlands

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  • Maaike van Zon,

    Affiliation: Division of Cell Biology-B6, Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital (NKI-AVL), Amsterdam, the Netherlands

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  • Brigitte Wever,

    Affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, the Netherlands

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  • Sander R. Piersma,

    Affiliation: Department of Medical Oncology, VU University Medical Centre, Amsterdam, the Netherlands

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  • Connie R. Jiménez,

    Affiliation: Department of Medical Oncology, VU University Medical Centre, Amsterdam, the Netherlands

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  • Mamadou Daffé,

    Affiliation: CNRS, Institut de Pharmacologie et de Biologie Structurale, Département Mécanismes Moléculaires des Infections Mycobactériennes, Toulouse, France

    Current address: Université de Toulouse (Toulouse III), Toulouse, France

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  • Ben J. Appelmelk,

    Affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, the Netherlands

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  • Wilbert Bitter,

    Affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, the Netherlands

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  • Nicole van der Wel mail,

    n.vd.wel@nki.nl (NvdW); p.peters@nki.nl (PJP)

    Affiliation: Division of Cell Biology-B6, Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital (NKI-AVL), Amsterdam, the Netherlands

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  • Peter J. Peters mail

    n.vd.wel@nki.nl (NvdW); p.peters@nki.nl (PJP)

    Affiliations: Division of Cell Biology-B6, Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital (NKI-AVL), Amsterdam, the Netherlands, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands

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  • Published: March 05, 2010
  • DOI: 10.1371/journal.ppat.1000794

About the Authors

Musa Sani, Jason Pierson, Karin de Punder, Maaike van Zon, Nicole van der Wel, Peter J. Peters
Division of Cell Biology-B6, Netherlands Cancer Institute–Antoni van Leeuwenhoek Hospital (NKI-AVL), Amsterdam, the Netherlands
Edith N. G. Houben, Jeroen Geurtsen, Brigitte Wever, Ben J. Appelmelk, Wilbert Bitter
Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, the Netherlands
Sander R. Piersma, Connie R. Jiménez
Department of Medical Oncology, VU University Medical Centre, Amsterdam, the Netherlands
Mamadou Daffé
CNRS, Institut de Pharmacologie et de Biologie Structurale, Département Mécanismes Moléculaires des Infections Mycobactériennes, Toulouse, France
Peter J. Peters
Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MS ENGH JG MD BJA WB NvdW PJP. Performed the experiments: MS ENGH JG JP KdP MvZ BW SRP CRJ. Analyzed the data: MS ENGH JG KdP NvdW. Wrote the paper: MS ENGH JG SRP MD BJA WB NvdW PJP.