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Research Article

Complement Receptor 1 Is a Sialic Acid-Independent Erythrocyte Receptor of Plasmodium falciparum

  • Carmenza Spadafora,

    Affiliations: Department of Medicine, the Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America, Department of Cellular Injury, Division of Military Casualty Research, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología-AIP (INDICASAT-AIP), Ciudad del Saber, Clayton, Panamá

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  • Gordon A. Awandare equal contributor,

    equal contributor Contributed equally to this work with: Gordon A. Awandare, Karen M. Kopydlowski

    Affiliation: Division of Malaria Vaccine Development, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America

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  • Karen M. Kopydlowski equal contributor,

    equal contributor Contributed equally to this work with: Gordon A. Awandare, Karen M. Kopydlowski

    Affiliation: Department of Parasitology, Division of Experimental Therapeutics, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America

    Current address: US Army Medical Materiel Development Activity (USAMMDA), Fort Detrick, Maryland, United States of America

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  • Jozsef Czege,

    Affiliation: Biomedical Instrumentation Center, the Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America

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  • J. Kathleen Moch,

    Affiliation: Department of Parasitology, Division of Experimental Therapeutics, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America

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  • Robert W. Finberg,

    Affiliation: Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

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  • George C. Tsokos,

    Affiliations: Department of Medicine, the Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America, Department of Cellular Injury, Division of Military Casualty Research, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America

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  • José A. Stoute mail

    jstoute@psu.edu

    Affiliations: Department of Medicine, the Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America, Department of Cellular Injury, Division of Military Casualty Research, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America, Department of Medicine, Division of Infectious Diseases and Epidemiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, United States of America

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  • Published: June 17, 2010
  • DOI: 10.1371/journal.ppat.1000968

About the Authors

Carmenza Spadafora, George C. Tsokos, José A. Stoute
Department of Medicine, the Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America
Carmenza Spadafora, George C. Tsokos, José A. Stoute
Department of Cellular Injury, Division of Military Casualty Research, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
Carmenza Spadafora
Instituto de Investigaciones Científicas y Servicios de Alta Tecnología-AIP (INDICASAT-AIP), Ciudad del Saber, Clayton, Panamá
Gordon A. Awandare
Division of Malaria Vaccine Development, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
Karen M. Kopydlowski, J. Kathleen Moch
Department of Parasitology, Division of Experimental Therapeutics, the Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
Jozsef Czege
Biomedical Instrumentation Center, the Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America
Robert W. Finberg
Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
George C. Tsokos
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
José A. Stoute
Department of Medicine, Division of Infectious Diseases and Epidemiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, United States of America

Corresponding Author

Email: jstoute@psu.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: CS GAA KMK GCT JAS. Analyzed the data: JAS. Contributed reagents/materials/analysis tools: JKM RWF. Wrote the paper: JAS. Performed all invasion assays with microscopic readout, prepared cultures for immunofluorescent microscopy, and carried out flow cytometry for CR1: CS. Edited the manuscript: CS GAA KMK. Carried out all the invasion assays with flow cytometry readout including experiments involving wild strains: GAA. Prepared cells for and carried out immunofluorescent microscopy: KMK. Performed immunofluorescent microscopy and processed data files: JC. Maintained malaria cultures for experiments: JKM. Contributed human CR1 transgenic mouse strain: RWF. Posed the initial hypotheses, created an outline of the design of the experiments, performed the microsphere binding and immunofluorescent assays, and carried out the CR1 immunoprecipitation: JAS.