Advertisement
Research Article

Biological and Structural Characterization of a Host-Adapting Amino Acid in Influenza Virus

  • Shinya Yamada,

    Affiliation: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan

    X
  • Masato Hatta,

    Affiliation: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Bart L. Staker,

    Affiliations: Emerald BioStructures, Inc., Bainbridge Island, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease, Washington, United States of America

    X
  • Shinji Watanabe,

    Affiliation: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Masaki Imai,

    Affiliation: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Kyoko Shinya,

    Affiliation: Department of Microbiology and Infectious Diseases, Kobe University, Hyogo, Japan

    X
  • Yuko Sakai-Tagawa,

    Affiliation: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan

    X
  • Mutsumi Ito,

    Affiliation: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan

    X
  • Makoto Ozawa,

    Affiliations: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America, Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan

    X
  • Tokiko Watanabe,

    Affiliation: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Saori Sakabe,

    Affiliations: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan, ERATO Infection-Induced Host Responses Project, Saitama, Japan

    X
  • Chengjun Li,

    Affiliation: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Jin Hyun Kim,

    Affiliation: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Peter J. Myler,

    Affiliations: Seattle Structural Genomics Center for Infectious Disease, Washington, United States of America, Seattle Biomedical Research Institute, Seattle, Washington, United States of America, Departments of Global Health and Medical Education & Biomedical Informatics, University of Washington, Seattle, Washington, United States of America

    X
  • Isabelle Phan,

    Affiliations: Seattle Structural Genomics Center for Infectious Disease, Washington, United States of America, Seattle Biomedical Research Institute, Seattle, Washington, United States of America

    X
  • Amy Raymond,

    Affiliations: Emerald BioStructures, Inc., Bainbridge Island, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease, Washington, United States of America

    X
  • Eric Smith,

    Affiliations: Emerald BioStructures, Inc., Bainbridge Island, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease, Washington, United States of America

    X
  • Robin Stacy,

    Affiliations: Seattle Structural Genomics Center for Infectious Disease, Washington, United States of America, Seattle Biomedical Research Institute, Seattle, Washington, United States of America

    X
  • Chairul A. Nidom,

    Affiliations: Faculty of Veterinary Medicine, Tropical Disease Centre, Airlangga University, Surabaya, Indonesia, Collaborating Research Center-Emerging and Reemerging Infectious Diseases, Tropical Disease Centre, Airlangga University, Surabaya, Indonesia

    X
  • Simon M. Lank,

    Affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Roger W. Wiseman,

    Affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Benjamin N. Bimber,

    Affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • David H. O'Connor,

    Affiliations: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America, Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Gabriele Neumann,

    Affiliation: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America

    X
  • Lance J. Stewart mail,

    kawaokay@svm.vetmed.wisc.edu (YK, for virological research aspects); lstewart@embios.com (LJS, for structural research aspects)

    Affiliations: Emerald BioStructures, Inc., Bainbridge Island, Washington, United States of America, Seattle Structural Genomics Center for Infectious Disease, Washington, United States of America

    X
  • Yoshihiro Kawaoka mail

    kawaokay@svm.vetmed.wisc.edu (YK, for virological research aspects); lstewart@embios.com (LJS, for structural research aspects)

    Affiliations: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan, Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America, Department of Microbiology and Infectious Diseases, Kobe University, Hyogo, Japan, Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan, ERATO Infection-Induced Host Responses Project, Saitama, Japan, Creative Research Initiative, Sousei, Hokkaido University, Sapporo, Japan

    X
  • Published: August 05, 2010
  • DOI: 10.1371/journal.ppat.1001034

About the Authors

Shinya Yamada, Yuko Sakai-Tagawa, Mutsumi Ito, Saori Sakabe, Yoshihiro Kawaoka
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Masato Hatta, Shinji Watanabe, Masaki Imai, Makoto Ozawa, Tokiko Watanabe, Chengjun Li, Jin Hyun Kim, Gabriele Neumann, Yoshihiro Kawaoka
Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America
Bart L. Staker, Amy Raymond, Eric Smith, Lance J. Stewart
Emerald BioStructures, Inc., Bainbridge Island, Washington, United States of America
Bart L. Staker, Peter J. Myler, Isabelle Phan, Amy Raymond, Eric Smith, Robin Stacy, Lance J. Stewart
Seattle Structural Genomics Center for Infectious Disease, Washington, United States of America
Kyoko Shinya, Yoshihiro Kawaoka
Department of Microbiology and Infectious Diseases, Kobe University, Hyogo, Japan
Makoto Ozawa, Yoshihiro Kawaoka
Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Saori Sakabe, Yoshihiro Kawaoka
ERATO Infection-Induced Host Responses Project, Saitama, Japan
Peter J. Myler, Isabelle Phan, Robin Stacy
Seattle Biomedical Research Institute, Seattle, Washington, United States of America
Peter J. Myler
Departments of Global Health and Medical Education & Biomedical Informatics, University of Washington, Seattle, Washington, United States of America
Chairul A. Nidom
Faculty of Veterinary Medicine, Tropical Disease Centre, Airlangga University, Surabaya, Indonesia
Chairul A. Nidom
Collaborating Research Center-Emerging and Reemerging Infectious Diseases, Tropical Disease Centre, Airlangga University, Surabaya, Indonesia
Simon M. Lank, Roger W. Wiseman, Benjamin N. Bimber, David H. O'Connor
Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America
David H. O'Connor
Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America
Yoshihiro Kawaoka
Creative Research Initiative, Sousei, Hokkaido University, Sapporo, Japan

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: M. Hatta, B. Staker, S. Watanabe, T. Watanabe, P. Myler, I. Phan, A. Raymond, E. Smith, R. Stacy, S. Lank, R. Wiseman, B. Bimber, D. O'Connor, L. Stewart, Y. Kawaoka. Performed the experiments: S. Yamada, M. Hatta, B. Staker, M. Imai, K. Shinya, Y. Sakai-Tagawa, M. Ito, M. Ozawa, T. Watanabe, S. Sakabe, C. Li, J. Kim, P. Myler, I. Phan, A. Raymond, E. Smith, R. Stacy, S. Lank, R. Wiseman, B. Bimber, D. O'Connor, L. Stewart. Analyzed the data: B. Staker, P. Myler, I. Phan, A. Raymond, E. Smith, R. Stacy, S. Lank, R. Wiseman, B. Bimber, D. O'Connor, L. Stewart, Y. Kawaoka. Contributed reagents/materials/analysis tools: C. Nidom. Wrote the paper: G. Neumann, Y. Kawaoka.