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Research Article

Structural Basis of HIV-1 Neutralization by Affinity Matured Fabs Directed against the Internal Trimeric Coiled-Coil of gp41

  • Elena Gustchina equal contributor,

    equal contributor Contributed equally to this work with: Elena Gustchina, Mi Li, John M. Louis

    Affiliation: Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Mi Li equal contributor,

    equal contributor Contributed equally to this work with: Elena Gustchina, Mi Li, John M. Louis

    Affiliations: Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland, United States of America, Basic Research Program, SAIC-Frederick, Frederick, Maryland, United States of America

    X
  • John M. Louis equal contributor,

    equal contributor Contributed equally to this work with: Elena Gustchina, Mi Li, John M. Louis

    Affiliation: Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • D. Eric Anderson,

    Affiliation: Proteomics and Mass Spectrometry Facility, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • John Lloyd,

    Affiliation: Proteomics and Mass Spectrometry Facility, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Christian Frisch,

    Affiliation: AbD Serotec, MorphoSys AG, Martinsried, Germany

    X
  • Carole A. Bewley,

    Affiliation: Laboratory of Biorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Alla Gustchina mail,

    mariusc@mail.nih.gov (GMC); wlodawer@nih.gov (AW); gustchia@mail.nih.gov (AG)

    Affiliation: Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland, United States of America

    X
  • Alexander Wlodawer mail,

    mariusc@mail.nih.gov (GMC); wlodawer@nih.gov (AW); gustchia@mail.nih.gov (AG)

    Affiliation: Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland, United States of America

    X
  • G. Marius Clore mail

    mariusc@mail.nih.gov (GMC); wlodawer@nih.gov (AW); gustchia@mail.nih.gov (AG)

    Affiliation: Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Published: November 11, 2010
  • DOI: 10.1371/journal.ppat.1001182

About the Authors

Elena Gustchina, John M. Louis, G. Marius Clore
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
Mi Li, Alla Gustchina, Alexander Wlodawer
Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland, United States of America
Mi Li
Basic Research Program, SAIC-Frederick, Frederick, Maryland, United States of America
D. Eric Anderson, John Lloyd
Proteomics and Mass Spectrometry Facility, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
Christian Frisch
AbD Serotec, MorphoSys AG, Martinsried, Germany
Carole A. Bewley
Laboratory of Biorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: JML CAB AG AW GMC. Performed the experiments: EG ML JML DEA JL. Analyzed the data: EG ML JML DEA JL AG AW GMC. Contributed reagents/materials/analysis tools: CF. Wrote the paper: EG CAB AG AW GMC.