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Research Article

Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays

  • Jason M. Wilham,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Christina D. Orrú,

    Affiliations: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America, Department of Biomedical Sciences and Technologies, University of Cagliari, Monserrato, Italy

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  • Richard A. Bessen,

    Affiliation: Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America

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  • Ryuichiro Atarashi,

    Affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Kyushu, Japan

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  • Kazunori Sano,

    Affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Kyushu, Japan

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  • Brent Race,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Kimberly D. Meade-White,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Lara M. Taubner,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Andrew Timmes,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Byron Caughey mail

    BCAUGHEY@niaid.nih.gov

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Published: December 02, 2010
  • DOI: 10.1371/journal.ppat.1001217

About the Authors

Jason M. Wilham, Christina D. Orrú, Brent Race, Kimberly D. Meade-White, Lara M. Taubner, Andrew Timmes, Byron Caughey
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America
Christina D. Orrú
Department of Biomedical Sciences and Technologies, University of Cagliari, Monserrato, Italy
Richard A. Bessen
Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America
Ryuichiro Atarashi, Kazunori Sano
Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Kyushu, Japan

Corresponding Author

Email: BCAUGHEY@niaid.nih.gov

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: JMW CDO RAB RA KS BC. Performed the experiments: JMW CDO RAB BR KDMW LMT AT. Analyzed the data: JMW RAB. Contributed reagents/materials/analysis tools: JMW RAB. Wrote the paper: JMW BC.