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Research Article

Respiratory Syncytial Virus Interferon Antagonist NS1 Protein Suppresses and Skews the Human T Lymphocyte Response

  • Shirin Munir,

    Affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

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  • Philippa Hillyer,

    Affiliation: Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, Maryland, United States of America

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  • Cyril Le Nouën,

    Affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

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  • Ursula J. Buchholz,

    Affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

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  • Ronald L. Rabin,

    Affiliation: Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, Maryland, United States of America

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  • Peter L. Collins,

    Affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

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  • Alexander Bukreyev mail

    alexander.bukreyev@utmb.edu

    Affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

    Current address: Galveston National Laboratory, Center for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America

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  • Published: April 21, 2011
  • DOI: 10.1371/journal.ppat.1001336

About the Authors

Shirin Munir, Cyril Le Nouën, Ursula J. Buchholz, Peter L. Collins, Alexander Bukreyev
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
Philippa Hillyer, Ronald L. Rabin
Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, Maryland, United States of America

Corresponding Author

Email: alexander.bukreyev@utmb.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SM PLC AB. Performed the experiments: SM. Analyzed the data: SM PH CLN UJB RLR PLC AB. Contributed reagents/materials/analysis tools: PH RLR PLC. Wrote the paper: SM PLC AB. Contributed critically to this work; developed the co-culture protocol, designed flow cytometry panels, optimized the polychromatic flow cytometry staining panels and participated critically in data analysis by designing initial gating strategy, ensuring proper fluorochrome compensation and providing guidance on FlowJo and SPICE analyses: PH.