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Research Article

Engineering HIV-Resistant Human CD4+ T Cells with CXCR4-Specific Zinc-Finger Nucleases

  • Craig B. Wilen,

    Affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

    X
  • Jianbin Wang,

    Affiliation: Sangamo BioSciences, Richmond, California, United States of America

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  • John C. Tilton,

    Affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

    Current address: Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, Ohio, United States of America.

    X
  • Jeffrey C. Miller,

    Affiliation: Sangamo BioSciences, Richmond, California, United States of America

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  • Kenneth A. Kim,

    Affiliation: Sangamo BioSciences, Richmond, California, United States of America

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  • Edward J. Rebar,

    Affiliation: Sangamo BioSciences, Richmond, California, United States of America

    X
  • Scott A. Sherrill-Mix,

    Affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • Sean C. Patro,

    Affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • Anthony J. Secreto,

    Affiliation: Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • Andrea P. O. Jordan,

    Affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • Gary Lee,

    Affiliation: Sangamo BioSciences, Richmond, California, United States of America

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  • Joshua Kahn,

    Affiliation: Sangamo BioSciences, Richmond, California, United States of America

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  • Pyone P. Aye,

    Affiliation: Divisions of Regenerative Medicine and Comparative Pathology, Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, United States of America

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  • Bruce A. Bunnell,

    Affiliation: Divisions of Regenerative Medicine and Comparative Pathology, Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, United States of America

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  • Andrew A. Lackner,

    Affiliation: Divisions of Regenerative Medicine and Comparative Pathology, Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, United States of America

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  • James A. Hoxie,

    Affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • Gwenn A. Danet-Desnoyers,

    Affiliation: Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • Frederic D. Bushman,

    Affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • James L. Riley,

    Affiliation: Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • Philip D. Gregory,

    Affiliation: Sangamo BioSciences, Richmond, California, United States of America

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  • Carl H. June,

    Affiliation: Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

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  • Michael C. Holmes,

    Affiliation: Sangamo BioSciences, Richmond, California, United States of America

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  • Robert W. Doms mail

    doms@mail.med.upenn.edu

    Affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

    X
  • Published: April 14, 2011
  • DOI: 10.1371/journal.ppat.1002020

About the Authors

Craig B. Wilen, John C. Tilton, Scott A. Sherrill-Mix, Sean C. Patro, Andrea P. O. Jordan, James A. Hoxie, Frederic D. Bushman, Robert W. Doms
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
Jianbin Wang, Jeffrey C. Miller, Kenneth A. Kim, Edward J. Rebar, Gary Lee, Joshua Kahn, Philip D. Gregory, Michael C. Holmes
Sangamo BioSciences, Richmond, California, United States of America
Anthony J. Secreto, Gwenn A. Danet-Desnoyers
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
Pyone P. Aye, Bruce A. Bunnell, Andrew A. Lackner
Divisions of Regenerative Medicine and Comparative Pathology, Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, United States of America
James L. Riley, Carl H. June
Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America

Corresponding Author

Email: doms@mail.med.upenn.edu

Competing Interests

A number of the authors are employed by Sangamo Biosciences. The work was funded by NIH.

Author Contributions

Conceived and designed the experiments: CBW JW JCT EJR SCP JAH GADD JLR PDG CHJ MCH RWD. Performed the experiments: CBW JW KAK SCP AJS APOJ. Analyzed the data: CBW JW JCM SCP. Contributed reagents/materials/analysis tools: SASM GL JK PPA BAB AAL FDB. Wrote the paper: CBW RWD.