Advertisement
Research Article

13C Metabolic Flux Analysis Identifies an Unusual Route for Pyruvate Dissimilation in Mycobacteria which Requires Isocitrate Lyase and Carbon Dioxide Fixation

  • Dany J. V. Beste,

    Affiliation: School of Biomedical and Molecular Sciences, University of Surrey, Guildford, United Kingdom

    X
  • Bhushan Bonde,

    Affiliation: School of Biomedical and Molecular Sciences, University of Surrey, Guildford, United Kingdom

    X
  • Nathaniel Hawkins,

    Affiliation: Rothamsted Research, National Centre for Plant and Microbial Metabolomics, Harpenden, Herts, United Kingdom

    X
  • Jane L. Ward,

    Affiliation: Rothamsted Research, National Centre for Plant and Microbial Metabolomics, Harpenden, Herts, United Kingdom

    X
  • Michael H. Beale,

    Affiliation: Rothamsted Research, National Centre for Plant and Microbial Metabolomics, Harpenden, Herts, United Kingdom

    X
  • Stephan Noack,

    Affiliation: Forschungszentrum Jülich GmbH, Institute of Bio- and Geosciences 1: Biotechnology 2, Jülich, Germany

    X
  • Katharina Nöh,

    Affiliation: Forschungszentrum Jülich GmbH, Institute of Bio- and Geosciences 1: Biotechnology 2, Jülich, Germany

    X
  • Nicholas J. Kruger,

    Affiliation: Department of Plant Sciences, University of Oxford, Oxford, United Kingdom

    X
  • R. George Ratcliffe,

    Affiliation: Department of Plant Sciences, University of Oxford, Oxford, United Kingdom

    X
  • Johnjoe McFadden mail

    j.mcfadden@surrey.ac.uk

    Affiliation: School of Biomedical and Molecular Sciences, University of Surrey, Guildford, United Kingdom

    X
  • Published: July 21, 2011
  • DOI: 10.1371/journal.ppat.1002091

About the Authors

Dany J. V. Beste, Bhushan Bonde, Johnjoe McFadden
School of Biomedical and Molecular Sciences, University of Surrey, Guildford, United Kingdom
Nathaniel Hawkins, Jane L. Ward, Michael H. Beale
Rothamsted Research, National Centre for Plant and Microbial Metabolomics, Harpenden, Herts, United Kingdom
Stephan Noack, Katharina Nöh
Forschungszentrum Jülich GmbH, Institute of Bio- and Geosciences 1: Biotechnology 2, Jülich, Germany
Nicholas J. Kruger, R. George Ratcliffe
Department of Plant Sciences, University of Oxford, Oxford, United Kingdom

Corresponding Author

Email: j.mcfadden@surrey.ac.uk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: DJVB JM. Performed the experiments: DJVB NH. Analyzed the data: JM DJVB JLW SN KN. Contributed reagents/materials/analysis tools: KN SN BB MHB NJK RGR. Wrote the paper: DJVB JM RGR NJK SN KN.