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Research Article

Structures of Merkel Cell Polyomavirus VP1 Complexes Define a Sialic Acid Binding Site Required for Infection

  • Ursula Neu equal contributor,

    equal contributor Contributed equally to this work with: Ursula Neu, Holger Hengel

    Affiliation: Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany

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  • Holger Hengel equal contributor,

    equal contributor Contributed equally to this work with: Ursula Neu, Holger Hengel

    Affiliation: Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany

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  • Bärbel S. Blaum,

    Affiliations: Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany, Department of Chemistry, University of Luebeck, Luebeck, Germany

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  • Rachel M. Schowalter,

    Affiliation: Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland, United States of America

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  • Dennis Macejak,

    Affiliation: Department of Molecular, Cellular, and Developmental Biology, and the Biofrontiers Institute, University of Colorado at Boulder, Boulder, Colorado, United States of America

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  • Michel Gilbert,

    Affiliation: National Research Council Canada, Institute for Biological Sciences, Glycobiology Program, Ottawa, Ontario, Canada

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  • Warren W. Wakarchuk,

    Affiliation: National Research Council Canada, Institute for Biological Sciences, Glycobiology Program, Ottawa, Ontario, Canada

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  • Akihiro Imamura,

    Affiliations: Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan, Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan

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  • Hiromune Ando,

    Affiliations: Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan, Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan

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  • Makoto Kiso,

    Affiliations: Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan, Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan

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  • Niklas Arnberg,

    Affiliation: Division of Virology, Department of Clinical Microbiology, Umeå University, Umeå, Sweden

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  • Robert L. Garcea,

    Affiliation: Department of Molecular, Cellular, and Developmental Biology, and the Biofrontiers Institute, University of Colorado at Boulder, Boulder, Colorado, United States of America

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  • Thomas Peters,

    Affiliation: Department of Chemistry, University of Luebeck, Luebeck, Germany

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  • Christopher B. Buck,

    Affiliation: Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland, United States of America

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  • Thilo Stehle mail

    thilo.stehle@uni-tuebingen.de

    Affiliations: Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America

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  • Published: July 26, 2012
  • DOI: 10.1371/journal.ppat.1002738

About the Authors

Ursula Neu, Holger Hengel, Bärbel S. Blaum, Thilo Stehle
Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany
Bärbel S. Blaum, Thomas Peters
Department of Chemistry, University of Luebeck, Luebeck, Germany
Rachel M. Schowalter, Christopher B. Buck
Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland, United States of America
Dennis Macejak, Robert L. Garcea
Department of Molecular, Cellular, and Developmental Biology, and the Biofrontiers Institute, University of Colorado at Boulder, Boulder, Colorado, United States of America
Michel Gilbert, Warren W. Wakarchuk
National Research Council Canada, Institute for Biological Sciences, Glycobiology Program, Ottawa, Ontario, Canada
Akihiro Imamura, Hiromune Ando, Makoto Kiso
Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan
Akihiro Imamura, Hiromune Ando, Makoto Kiso
Department of Applied Bioorganic Chemistry, Gifu University, Gifu, Japan
Niklas Arnberg
Division of Virology, Department of Clinical Microbiology, Umeå University, Umeå, Sweden
Thilo Stehle
Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America

Corresponding Author

Email: thilo.stehle@uni-tuebingen.de

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: UN HH BSB TP RMS CBB TS. Performed the experiments: UN HH BSB RMS. Analyzed the data: UN HH BSB RMS TP CBB TS. Contributed reagents/materials/analysis tools: DM MG WWW AI HA MK NA RLG TP. Wrote the paper: UN BSB RMS CBB TS.