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Research Article

Establishment of a Reverse Genetics System for Studying Human Bocavirus in Human Airway Epithelia

  • Qinfeng Huang equal contributor,

    equal contributor Contributed equally to this work with: Qinfeng Huang, Xuefeng Deng, Ziying Yan

    Affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America

    X
  • Xuefeng Deng equal contributor,

    equal contributor Contributed equally to this work with: Qinfeng Huang, Xuefeng Deng, Ziying Yan

    Affiliations: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America, College of Life Sciences, Central China Normal University, Wuhan, China

    X
  • Ziying Yan equal contributor,

    equal contributor Contributed equally to this work with: Qinfeng Huang, Xuefeng Deng, Ziying Yan

    Affiliation: Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, Iowa, United States of America

    X
  • Fang Cheng,

    Affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America

    X
  • Yong Luo,

    Affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America

    X
  • Weiran Shen,

    Affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America

    X
  • Diana C. M. Lei-Butters,

    Affiliation: Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, Iowa, United States of America

    X
  • Aaron Yun Chen,

    Affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America

    X
  • Yi Li,

    Affiliation: College of Life Sciences, Central China Normal University, Wuhan, China

    Current address: Department of Bioengineering, Wuhan Bioengineering Institute, Economic Development Zone of Yangluo, Wuhan, Hubei, China

    X
  • Liang Tang,

    Affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, United States of America

    X
  • Maria Söderlund-Venermo,

    Affiliation: Haartman Institute, Department of Virology, University of Helsinki, Helsinki, Finland

    X
  • John F. Engelhardt,

    Affiliation: Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, Iowa, United States of America

    X
  • Jianming Qiu mail

    jqiu@kumc.edu

    Affiliation: Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America

    X
  • Published: August 30, 2012
  • DOI: 10.1371/journal.ppat.1002899

About the Authors

Qinfeng Huang, Xuefeng Deng, Fang Cheng, Yong Luo, Weiran Shen, Aaron Yun Chen, Jianming Qiu
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, United States of America
Xuefeng Deng, Yi Li
College of Life Sciences, Central China Normal University, Wuhan, China
Ziying Yan, Diana C. M. Lei-Butters, John F. Engelhardt
Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, Iowa, United States of America
Liang Tang
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, United States of America
Maria Söderlund-Venermo
Haartman Institute, Department of Virology, University of Helsinki, Helsinki, Finland

Corresponding Author

Email: jqiu@kumc.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: Q.Huang X.Deng Z.Yan Y.Li L.Tang J.Qiu. Performed the experiments: Q.Huang X.Deng Z.Yan F.Cheng Y.Luo W.Shen D.C.M.Lei-Butters A.Y.Chen L.Tang. Analyzed the data: Q.Huang X.Deng Z.Yan L.Tang J.Qiu. Contributed reagents/materials/analysis tools: M.Söderlund-Venermo. Wrote the paper: Q.Huang X.Deng Z.Yan M.Söderlund-Venermo J.F.Engelhardt J.Qiu.