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Research Article

H1N1 2009 Pandemic Influenza Virus: Resistance of the I223R Neuraminidase Mutant Explained by Kinetic and Structural Analysis

  • Erhard van der Vries,

    Affiliation: Erasmus Medical Centre, Department of Virology, Rotterdam, The Netherlands

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  • Patrick J. Collins,

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Sebastien G. Vachieri,

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Xiaoli Xiong,

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Junfeng Liu,

    Affiliations: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom, MOA Key Laboratory of Plant Pathology, China Agricultural University, Beijing, People's Republic of China

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  • Philip A. Walker,

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Lesley F. Haire,

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Alan J. Hay,

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Martin Schutten,

    Affiliation: Erasmus Medical Centre, Department of Virology, Rotterdam, The Netherlands

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  • Albert D. M. E. Osterhaus,

    Affiliation: Erasmus Medical Centre, Department of Virology, Rotterdam, The Netherlands

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  • Steve R. Martin,

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Charles A. B. Boucher,

    Affiliation: Erasmus Medical Centre, Department of Virology, Rotterdam, The Netherlands

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  • John J. Skehel mail,

    skeheljj@nimr.mrc.ac.uk

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Steve J. Gamblin

    Affiliation: Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom

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  • Published: September 20, 2012
  • DOI: 10.1371/journal.ppat.1002914

About the Authors

Erhard van der Vries, Martin Schutten, Albert D. M. E. Osterhaus, Charles A. B. Boucher
Erasmus Medical Centre, Department of Virology, Rotterdam, The Netherlands
Patrick J. Collins, Sebastien G. Vachieri, Xiaoli Xiong, Junfeng Liu, Philip A. Walker, Lesley F. Haire, Alan J. Hay, Steve R. Martin, John J. Skehel, Steve J. Gamblin
Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom
Junfeng Liu
MOA Key Laboratory of Plant Pathology, China Agricultural University, Beijing, People's Republic of China

Corresponding Author

Email: skeheljj@nimr.mrc.ac.uk

Competing Interests

I have read the journal's policy and have the following conflicts: MS is consultant of ViroClinics Biosciences BV. MS is advisor for Hoffmann-La Roche, Inc. MS is advisor for Vertex Ltd. ADMEO is employe of ViroClinics Biosciences BV. ADMEO is chief scientific officer of ViroClinics Biosciences BV. ADMEO, MS, EVDV and CABB participate in the IRIS study sponsored by Hoffmann-La Roche, Inc. This does not alter our adherence to all PLoS Pathogens policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: EVDV PJC SGV XX ADMEO CABB JJS SJG. Performed the experiments: EVDV PJC SGV XX JL PAW LFH SRM JJS SJG. Analyzed the data: EVDV PJC SGV XX JL PAW LFH SRM JJS SJG. Contributed reagents/materials/analysis tools: EVDV PJC SBV XX JL PAW LFH AJH MS SRM CABB JJS SJG. Wrote the paper: EVDV JJS SJG CABB.