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Fluorescence in a tobacco plant infected with a recombinant plant negative strand RNA virus carrying the GFP gene.

Wang Qiang and Colleagues have used Sonchus yellow net rhabdovirus (SYNV) to develop the first reverse genetics system reported for a plant negative strand RNA virus. The SYNV genome was cloned and a derivative expressing GFP (rSYNV-GFP) was constructed and used to infect plants. The image shows GFP fluorescence against a red chloroplast autofluorescence background in a Nicotiana benthamiana plant systemically infected with rSYNV-GFP. Li et al.

Image Credit: Li et al.