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Research Article

Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays

  • Jason M. Wilham,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Christina D. OrrĂº,

    Affiliations: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America, Department of Biomedical Sciences and Technologies, University of Cagliari, Monserrato, Italy

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  • Richard A. Bessen,

    Affiliation: Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America

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  • Ryuichiro Atarashi,

    Affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Kyushu, Japan

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  • Kazunori Sano,

    Affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Kyushu, Japan

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  • Brent Race,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Kimberly D. Meade-White,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Lara M. Taubner,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Andrew Timmes,

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Byron Caughey mail

    BCAUGHEY@niaid.nih.gov

    Affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America

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  • Published: December 02, 2010
  • DOI: 10.1371/journal.ppat.1001217

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