DNA methylation regulates gene expression in many organisms. In eukaryotes, DNA methylation is associated with gene repression, while it exerts both activating and repressive effects in the Proteobacteria through largely locus-specific mechanisms. Here, we identify a critical DNA methyltransferase in M. tuberculosis, which we term MamA. MamA creates N6-methyladenine in a six base pair recognition sequence present in approximately 2,000 copies on each strand of the genome. Loss of MamA reduces the expression of a number of genes. Each has a MamA site located at a conserved position relative to the sigma factor −10 binding site and transcriptional start site, suggesting that MamA modulates their expression through a shared, not locus-specific, mechanism. While strains lacking MamA grow normally in vitro, they are attenuated in hypoxic conditions, suggesting that methylation promotes survival in discrete host microenvironments. Interestingly, we demonstrate strikingly different patterns of DNA methyltransferase activity in different lineages of M. tuberculosis, which have been associated with preferences for distinct host environments and different disease courses in humans. Thus, MamA is the major functional adenine methyltransferase in M. tuberculosis strains of the Euro-American lineage while strains of the Beijing lineage harbor a point mutation that largely inactivates MamA but possess a second functional DNA methyltransferase. Our results indicate that MamA influences gene expression in M. tuberculosis and plays an important but strain-specific role in fitness during hypoxia.
Tuberculosis is a disease with a devastating impact on public health, killing over 1.5 million people each year around the globe. Tuberculosis is caused by the bacterium Mycobacterium tuberculosis, which over millennia has evolved the ability to survive and persist for decades in the harsh environment inside its human host. Regulation of gene expression is critical for adaptation to stressful conditions. To successfully tackle M. tuberculosis, we therefore need to understand how it regulates its genes and responds to environmental stressors. In this work, we report the first investigation of the role of DNA methylation in gene regulation and stress response in M. tuberculosis. We have found that DNA methylation is important for survival of hypoxia, a stress condition present in human infections, and furthermore that DNA methylation affects the expression of several genes. In contrast to methylation-regulation systems reported in other bacteria, in which the effects of methylation vary from one gene to the next, M. tuberculosis appears to use a concerted mechanism to influence multiple genes. Our findings identify a novel mechanism by which M. tuberculosis modulates gene expression in response to stress.
Citation: Shell SS, Prestwich EG, Baek S-H, Shah RR, Sassetti CM, et al. (2013) DNA Methylation Impacts Gene Expression and Ensures Hypoxic Survival of Mycobacterium tuberculosis. PLoS Pathog 9(7): e1003419. doi:10.1371/journal.ppat.1003419
Editor: William R. Bishai, Johns Hopkins School of Medicine, United States of America
Received: July 5, 2012; Accepted: April 30, 2013; Published: July 4, 2013
Copyright: © 2013 Shell et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the National Institutes of Health New Innovator Award 1DP2OD001378-01 (SMF) and award AI064282 (CMS), Ruth Kirschstein award 5F32AI085911-02 from NIAID (SSS), NIEHS Training Grant in Environmental Toxicology award 5T32-ES007020-34 (EGP), the Howard Hughes Medical Institute (CMS and SMF), the Heiser Program for Research in Leprosy and Tuberculosis (SSS), the NIH Loan Repayment Program (SSS) and the Singapore-MIT Alliance for Research and Technology (PCD), the Doris Duke Charitable Foundation (SMF), the Burroughs Wellcome Fund (SMF), and the Hood Foundation (SMF). Mass spectrometry studies were performed in the Bioanalytical Facilities Core of the MIT Center for Environmental Health Sciences, which is supported by NIEHS grantES002109. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Mycobacterium tuberculosis is a pathogen of tremendous global significance, causing 9 million cases of tuberculosis annually and latently infecting up to a third of the world's population . Untreated, M. tuberculosis can persist for decades in the infected host. Over such timescales, the bacterium must tune gene expression patterns to match conditions in the host environment, including hypoxia, nutrient deprivation, and low pH, and maintain these adaptations over long periods of time.
How might M. tuberculosis durably maintain gene expression patterns? While eukaryotes use a variety of mechanisms to heritably ensure expression states, DNA methylation is the only known mechanism by which prokaryotes might achieve epigenetic inheritance. Both adenine and cytosine can be methylated in DNA, resulting in N6-methyladenine, N4-methylcytosine, and 5-methylcytosine (accurately termed N6-methyl-2′deoxyadenosine, N4-methyl-2′deoxycytidine, and 5-methyl-2′deoxycytidine, and abbreviated here as N6-MdA, N4-MdC, and 5-MdC, respectively). Cytosine methylation is an important mechanism of repressing gene expression in higher eukaryotes and recent reports suggest that 5-MdC has regulatory roles in prokaryotes , . However, in prokaryotes N6-MdA is the best-characterized epigenetic regulator of gene expression –.
Regulation of gene expression by adenine methylation has been described mainly in the Proteobacteria where it is primarily mediated by the Dam methyltransferase in the Gammaproteobacteria and CcrM in the Alphaproteobacteria, although other methyltransferases of unknown function have been identified , . Dam-mediated methylation has pleiotropic roles that include directing DNA mismatch repair, suppressing transposition, and regulating genes involved in cell cycle timing and antigenic variation –, –. In Escherichia coli, genetic disruption of dam causes a modest growth defect , an increased mutation rate , , and numerous gene expression changes –. Some of these expression changes result directly from the methylation state of a given promoter, but most seem to reflect the downstream consequences of cell cycle changes and perturbed DNA repair –, , –, –. Even where Dam methylation has been shown to regulate gene expression directly, the mechanistic details are highly locus-specific , , , . There are several known transcriptional repressors that bind DNA in a methylation state dependent manner. Methylation may permit or prevent repressor binding, depending on the repressor and the spatial relationship between the Dam site and other promoter elements. However, the pleiotropic roles of Dam methylation in cell cycle regulation and DNA repair make it difficult to distinguish between direct and indirect effects on gene expression. Furthermore, over half of the ORFs in the E. coli genome have two or more Dam sites in the 500 base pair region upstream , making the presence of Dam sites a poor indicator of Dam-mediated regulation.
Virulent M. tuberculosis has been reported to contain both N6-MdA and 5-MdC . However, there are no predicted dam or dcm homologues in the genome and canonical Dam and Dcm sites are not methylated , . Van Soolingen and colleagues identified a site in the lppC gene that was protected from restriction digest in clinical M. tuberculosis strains  and predicted this to be due to DNA methylation. However, nothing further was known about the mechanism or functional consequences of DNA methylation in M. tuberculosis.
Interestingly, the extent of lppC protection differed among strains from the different phylogeographic lineages of M. tuberculosis, with strains of the Beijing lineage showing reduced lppC protection compared to strains from other lineages . The various lineages of M. tuberculosis are associated with different epidemiological characteristics. Most notably, strains of the Beijing lineage appear to be increasing in prevalence globally, suggesting that this lineage has a competitive advantage in the modern world –. While the success of the Beijing lineage is likely multifactorial, some of its unique characteristics have been hypothesized to arise from differences in regulatory circuitry that may alter adaptation to specific host environments , –.
Based on these findings, we hypothesized that DNA methylation might regulate gene expression in M. tuberculosis, with functional significance in specific host environments or genetic contexts. We identify a methyltransferase, MamA (M.MtuHIII according to systematic DNA methyltransferase nomenclature ), and show that it methylates a six base pair sequence in the M. tuberculosis genome in a strain specific manner. We demonstrate that MamA methylation affects expression of several genes. Using a novel approach to map the transcriptional start sites of these genes we demonstrate that in each case, a methylation site overlaps with the sigma factor binding site in an identical configuration. Importantly, we show that loss of MamA reduces the ability of M. tuberculosis to survive in hypoxia, a stressor thought to mimic the environment that the bacterium encounters in the human host.
A putative methylation site exhibits strain-dependent variability in restriction digest susceptibility
In order to investigate the determinants of DNA methylation in the M. tuberculosis genome, we began by examining a site in the lppC gene that had been previously reported to be protected from restriction enzyme cleavage . Consistent with the published data, we confirmed that this site was largely protected from cleavage by PvuII in M. tuberculosis strains from the Euro-American lineage and the vaccine strain M. bovis BCG (Figure 1, A and B), but was fully susceptible to PvuII in strain HN878, a member of the Beijing lineage of M. tuberculosis (Figure 1, B and C). As the PvuII recognition sequence was present in all strains, it had been postulated that differential methylation was the most likely explanation for the variable PvuII cleavage . A 10 base pair sequence containing the PvuII recognition site was shown to be protected from PvuII cleavage ; methylation of the adenine residues within this sequence is expected to block PvuII cleavage  and the effects of cytosine methylation are unknown (Figure 1E).
Figure 1. MamA is a DNA methyltransferase that protects lppC from endonucleolytic cleavage.
(A) Southern blotting strategy to assess the status of a PvuII site near the 3′ end of lppC. Genomic DNA was digested with PvuII and analyzed by Southern blot with a probe hybridizing as shown. Fully cleaved DNA generates a 1.8 kb product, while protected DNA produces a 2.1 kb product. (B) DNA from the vaccine strain M. bovis BCG and from M. tuberculosis strains of the Euro-American lineage (Erdmann and CDC1551) is partially protected from PvuII cleavage while DNA from a Beijing lineage strain (HN878) is not. (C) Genetic deletion of mamA abrogates protection of lppC, while deletion of hsdM does not affect protection. (D) Protection is restored by complementation of a ΔmamA strain with an ectopic copy of mamA, but not by empty vector or mamAE270A. (E) Sequence context of the assayed PvuII site. Underlined bases are predicted to block PvuII if methylated.doi:10.1371/journal.ppat.1003419.g001
Rv3263 encodes the active DNA methyltransferase MamA
There are two predicted DNA methyltransferases encoded in the M. tuberculosis genome, neither of which is associated with a cognate restriction endonuclease. To determine if either of these methyltransferases was responsible for the DNA modification at the lppC site, we constructed unmarked deletion mutants of these genes in H37Rv, a commonly used lab strain of M. tuberculosis that belongs to the EuroAmerican lineage. Deletion of Rv3263 abolished protection of the lppC site from PvuII cleavage (Figure 1C). In contrast, deletion of hsdM did not affect protection of this site. Complementation of the Rv3263 deletion strain with an ectopic copy of the gene restored protection (Figure 1D and Figure S1). The Rv3263 gene product from H37Rv will be called M.MtuHIII according to standard DNA methyltransferase nomenclature . As systematic methyltransferase names are strain-specific, we have also chosen a generic name that can be applied to all M. tuberculosis strains. We therefore refer to Rv3263 and its gene product as mamA and MamA, respectively (Mycobacterial adenine methyltransferase). MamA is conserved in relatives of M. tuberculosis including M. bovis BCG (Figure 1), the pathogens M. leprae and M. avium, and the saprophyte M. smegmatis (TB Database, ).
Sequence trace comparison reveals a six base pair recognition site for adenine methylation by MamA
To identify the base that MamA methylates, we constructed an episomal plasmid containing the 10 base pair sequence sufficient to enable protection from PvuII cleavage and propagated the plasmid in both wildtype M. tuberculosis and the mamA deletion mutant. We then assessed the methylation status of the 10 base pair sequence using sequence trace comparison. This method is based on differing incorporation of dye terminator nucleotides complementary to methylated adenine or cytosine residues in conventional Sanger sequencing, allowing methylation status to be inferred by comparing sequencing traces from identical sequences of DNA propagated in the presence and absence of the methyltransferase , . The change in nucleotide incorporation depends on the methylated base in the template: N6-MdA results in increased incorporation of dideoxythymidine nucleotides yielding higher thymine peaks while 5-MdC and N4-MdC result in less and more dideoxyguanosine incorporation, respectively, and thus lower and higher guanine peaks , , . We propagated the plasmid in methylation-proficient and methylation-deficient M. tuberculosis and E. coli, then purified and sequenced it. Representative sequence traces are shown in Figure 2A. The thymine peak in position 5 of the top strand sequence showed increased intensity in plasmid isolated from the methylation-proficient M. tuberculosis strain H37Rv, relative to the equivalent peak in sequences of plasmid isolated from E. coli, H37Rv ΔMamA, and M. tuberculosis strain HN878. Similarly, the thymine peak in position 3 of the opposite strand was relatively higher in plasmid from H37Rv. Quantification of differences in peak area is shown in Figure S2. These alterations in relative peak height reflect increases in dideoxythymidine incorporation, suggesting presence of N6-MdA in the complementary templates isolated from H37Rv (Figure 2B).
Figure 2. Sequence trace comparison identifies the target base and minimal recognition sequence of MamA.
Plasmids containing putative MamA-recognition motifs were propagated in the indicated bacterial strains, isolated and sequenced. Sequence traces shown are representative of at least 2–3 biological replicates. (A) The 10 base pair sequence shown in Figure 1E supports methylation of one adenine on each strand in wildtype H37Rv, as evidenced by increased thymine peak areas relative to the identical sequence context in E. coli and methylation-deficient strains of M. tuberculosis. See Figure S2 for quantification of peak areas. (B) Schematic depiction of the positions of N6-methyladenine residues. (C) A six base pair core sequence is sufficient to direct MamA-mediated methylation (bold in panel B). See Table S1 for a complete list of tested sequences. (D) Positions of MamA recognition sequences are shown schematically on the 4.4 Mb M. tuberculosis genome.doi:10.1371/journal.ppat.1003419.g002
We also noted a reduction in the height of the guanine peak following the elevated thymine peak in H37Rv-derived DNA (Figure 2A, “top strand” and Figure S2A). This reflects decreased dideoxyguanosine incorporation and would be consistent with the presence of 5-MdC in the template, but bisulfite sequencing of H37Rv-derived plasmid indicated that no methylcytosine was present (data not shown). The peak height difference is therefore likely a result of the preceding N6-MdA causing an effective change in sequence context. Similar alterations in the incorporation of nucleotides neighboring the base complementary to the site of methylation have been observed previously , .
To determine the minimal recognition sequence required for methylation by MamA, we systematically mutated the 10 base pair sequence shown in Figure 2B and performed sequence trace comparison on the resulting plasmids. A central core of six base pairs (bold in Figure 2B) was sufficient to direct methylation in H37Rv (Figure 2C). Any further changes to this six base pair sequence abrogated methylation (Table S1). The MamA recognition site “CTGGAG” is predicted to be present in 1947 locations in the H37Rv genome. The sites are distributed across the genome, without any obvious skew with respect to the origin of replication (Figure 2D). Interestingly, there is a strong bias regarding the orientations of MamA sites relative to the coding strand within open reading frames. Of the 1816 times that MamA sites occur within annotated coding regions, the sequence reading “CTGGAG” is located on the coding strand in 1511 cases, while it is located on the non-coding strand in only 305 cases (p<0.0001, Chi square test with Yates correction). This may be at least partially a result of codon bias, as the codons “CTG” and “GAG” are both favored in M. tuberculosis while “CTC,” “TCC,” and “CCA” are all relatively disfavored . Two other bacterial DNA methyltransferases, M.GsuI and M.BpmI, are known to recognize an identical sequence to MamA; however, the roles of these enzymes are not known so they did not provide clues regarding the function of MamA (Rebase, ).
MamA is the predominant DNA methyltransferase in M. tuberculosis, H37Rv
To investigate the role of MamA within the broader DNA methylation landscape of M. tuberculosis, we defined the spectrum of methylated nucleobases in M. tuberculosis DNA using liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS). Genomic DNA was enzymatically digested to individual nucleosides and subject to LC- MS/MS to quantify N6-MdA, 5-MdC, N4-MdC, and 5-hydroxymethyl-2′deoxycytidine (5-HMdC). In DNA from wildtype H37Rv, N6-MdA occurred at a rate of 4.9+/−2.2 per 104 nucleotides (nts) (0.27% of adenosines) (Figure 3A), which would yield 2048+/−910 N6-MdA per strand in the 4.4 million bp genome, correlating well with the expected 1947 MamA sites (+/− denotes SD). Deletion of mamA reduced N6-MdA to less than five N6-MdA per genome strand. Taken together, these data indicate that MamA is the major adenine methytransferase active in H37Rv. Complementation of H37Rv ΔmamA with mamA restored N6-MdA to wildtype levels. 5-MdC, N4-MdC, and 5-HMdC were not detected in any of the M. tuberculosis strains (limits of detection were approximately five per 108 nts for 5-MdC and N4-MdC, and one per 105 nts for 5-HMdC). The absence of 5-MdC was somewhat surprising given that this modification has been previously reported in H37Rv , . However, it is consistent with the absence of a predicted cytosine methyltransferase in the genome.
Figure 3. Quantitation of total N6-MdA content in M. tuberculosis.
Genomic DNA from the indicated strains was digested to individual nucleosides and methylation content determined by liquid chromatography-coupled tandem mass spectrometry. Results are expressed as the amount of N6-MdA per nucleotide (left axis) and percentage of adenines that are methylated in each genome (right axis). Each represents at least three biological replicates. Outliers were removed using Grubbs criteria and error bars represent ± standard deviation. (A) Analysis of the contribution of wildtype and mutant forms of MamA to total adenine methylation levels in strain H37Rv. (B) Analysis of the contributions of MamA and HsdM to total adenine methylation levels in Euro-American (H37Rv) and Beijing (HN878) strain backgrounds.doi:10.1371/journal.ppat.1003419.g003
To confirm that MamA is itself a DNA methyltransferase (rather than an activator of other methyltransferases, for example), we made point mutations predicted to disrupt the MamA active site. MamA is predicted to be a Type II DNA methyltransferase with architecture of the gamma subtype , and is therefore homologous to the well-characterized methyltransferases M.EcoKI and M.TaqI. Biochemical studies of these and other adenine methyltransferases demonstrate that mutations of key residues in the S-adenosylmethionine binding site inhibit methyltransferase activity without disrupting overall protein structure –. We therefore mutated these key residues and expressed constructs encoding MamAN127D and MamAY130A in H37Rv ΔmamA. We then assessed the effects of these mutations on DNA methylation using LC-MS/MS analysis. Both mutants displayed little or no adenine methylation (Figure 3A), indicating that the predicted active site of MamA is critical to its ability to confer methylation.
MamA and HsdM are differentially active in Beijing lineage vs. Euro-American lineage strains
We sought to understand the loss of adenine methylation at the PvuII site in lppC in Beijing lineage strains of M. tuberculosis. Sequencing of mamA revealed that the Beijing lineage strain HN878, and all modern Beijing lineage strains for which genome sequences are publicly available, have a point mutation of nucleotide A809C causing the amino acid substitution Glu270Ala. While Glu270 is not located in the predicted active site, it is part of a conserved motif, suggesting that a non-conservative mutation to Ala could disrupt enzyme activity. Consistent with this hypothesis, mamAE270A failed to restore protection from PvuII cleavage in an H37Rv ΔmamA strain (Figure 1D). LC-MS/MS analysis revealed that H37Rv harboring mamAE270A had N6-MdA levels that were 50–100 fold lower than the wildtype parent (Figure 3A).
Interestingly, strain HN878 had only a 3-fold reduction in N6-MdA compared to H37Rv, despite harboring the mamAE270A allele (Figure 3A). This suggested that HN878 has a substantial amount of MamA-independent adenine methylation, in contrast to H37Rv. We therefore further examined the contributions of the two predicted DNA methyltransferases, MamA and HsdM, to total adenine methylation levels in the two strain backgrounds. In H37Rv and most members of the Euro-American lineage of M. tuberculosis, hsdM contains a mutation resulting in the amino acid change Pro306Leu in the active site, which is predicted to abolish HsdM activity , . Indeed, LC-MS/MS analysis of H37Rv ΔhsdM demonstrated that deletion of hsdM did not reduce levels of N6-MdA suggesting that in H37Rv, hsdM does not appreciably contribute to the N6-MdA content of the genome. Consistent with the idea that a Pro306Leu mutation is responsible for the lack of detectable HsdM activity in H37Rv, reintroduction of a wildtype Pro306 allele of hsdM to H37Rv ΔmamA significantly increased N6-MdA levels (Figure 3B). Since HN878 naturally encodes a wildtype Pro306 allele of hsdM, the excess N6-MdA in HN878 relative to H37Rv mamAE270A is likely to reflect greater HsdM activity in HN878 as compared to H37Rv.
We also predicted that complementing HN878 with a wildtype Glu270 allele of mamA would increase total N6-MdA levels. Interestingly, restoration of wildtype MamA to HN878 resulted in a quantitatively greater increase in N6-MdA than expected based on the effect of complementing H37Rv ΔmamA with the same construct expressing mamA (Figure 3). These data suggest that strain genetic background affects expression and/or activity of individual methyltransferases.
Global expression profiling reveals differential gene expression in ΔmamA strains
As DNA methylation regulates gene expression in other organisms, we sought to determine if MamA serves a similar function in M. tuberculosis. We used an Affymetrix microarray platform to perform global transcriptional profiling of triplicate log-phase cultures of wildtype H37Rv, ΔmamA, and complemented strains (Table S2 for complete dataset; GEO accession number GSE46432). Table 1 lists genes with expression differences of 1.5-fold or greater between wildtype H37Rv and either of the other two strains. Because we saw only a modest number of expression differences of limited magnitude, we felt that the microarray experiment was best used as a hypothesis-generating tool. Recognizing that small changes in a bulk expression assay may reflect larger changes in heterogeneous subpopulations of bacteria, we hypothesize that such apparently subtle changes might be functionally important. Several genes showed lower expression in ΔmamA compared to wildtype and complemented strains and had MamA sites in the region upstream of their annotated start codons (Table 1). These genes were considered to be candidates whose expression might be directly regulated by DNA methylation. Other genes showed altered expression only in the complemented strain relative to the wildtype and ΔmamA strains. These genes were located in the vicinity of the integrating complementation vector and their expression changes were thus likely be a result the strain construction strategy and not related to methylation status. Rv0102, Rv0142, corA, whiB7, and the Rv3083 operon were the strongest candidate methylation-affected genes. We therefore re-tested their expression levels by quantitative PCR (qPCR), using RNA derived from independent cultures, and confirmed that the ΔmamA strain had significantly reduced expression of Rv0102, Rv0142, corA, and whiB7 (Figure 4).
Figure 4. Several genes have lower expression levels in a ΔmamA strain.
Expression of each gene was determined by quantitative PCR in the indicated H37Rv-derived strains and is displayed as a relative value compared to expression of the housekeeping gene sigA in the same strain. Values shown are the mean of three technical replicates. Error bars denote standard deviation. (*) denotes P<0.05 compared to the wildtype and complemented strains (ANOVA with Tukey's post test). The wildtype and complemented strains were not significantly different from each other for any of the genes tested. The experiment was performed using RNA from different cultures than those used to prepare RNA for microarrays.doi:10.1371/journal.ppat.1003419.g004
Table 1. Genes with expression differences of 1.5-fold or greater in ΔmamA or complemented strains compared to the wildtype parent (H37Rv) in aerobic growth conditions.doi:10.1371/journal.ppat.1003419.t001
Transcriptional start site (TSS)-mapping suggests direct modulation of gene expression by MamA methylation
To understand how MamA affects gene expression, we mapped the transcriptional start sites (TSSs) of the qPCR-confirmed genes. We employed a novel strategy based on mRNA circularization in order to map TSSs quickly and accurately (Figure 5A). Total RNA preparations were subject to rRNA depletion and treated with a 5′polyphosphatase to convert 5′ triphosphate “caps” to 5′ monophosphates. The resulting mRNA-enriched samples were circularized by T4 RNA ligase to create molecules containing junctions between 5′ and 3′ ends. Linear cDNA molecules were synthesized from the circular templates by random priming. The 5′-3′ junctions were amplified by gene-specific primers and sequenced by a reverse primer annealing shortly downstream of the start codon. Because the 3′ ends of mRNAs are variable, the 5′-3′ junctions appeared as transitions from monomorphic to polymorphic sequence (Figure 5B). We validated this method by mapping the TSS of whiB1, and found that our method predicted a TSS identical to that identified by 5′ Rapid Amplification of cDNA Ends (Figure S3) .
Figure 5. Transcriptional start site (TSS)-mapping reveals a consistent spatial relationship between MamA sites and TSSs of methylation-affected genes.
TSSs in strain H37Rv were mapped by the strategy outlined in (A). mRNA was circularized before random-primed synthesis of cDNA. Dashes indicate the variable 3′ end of an mRNA. Gene-specific primers were then used to amplify and sequence 5′-3′ junctions. Junctions appear as transitions from clean to messy sequence due to the variable 3′ ends. (B) TSS-mapping sequence traces are shown for the four genes whose expression is reproducibly affected by MamA. MamA sites, putative sigma factor −10 binding sites, TSSs and ORFs are shown as indicated in the key.doi:10.1371/journal.ppat.1003419.g005
The TSSs of Rv0102, Rv0142, corA, and whiB7 were each located four or five base pairs downstream of a MamA methylation site (Figure 5B). In each case, the predicted sigma factor −10 binding site overlaps with the MamA site such that the last nucleotide of the −10 site is predicted to be methylated on the template strand while the nucleotide three base pairs downstream of the −10 site is predicted to be methylated on the non-template strand (Figure 5B). The conserved spatial relationship between methylation sites and sigma factor binding sites is striking and potentially suggestive of a shared regulatory paradigm among these genes.
mamA deletion reduces survival of M. tuberculosis in hypoxia
Given a role for MamA in influencing gene expression, we then sought to determine the functional consequences of losing MamA function. Because DNA methylation plays roles in cell cycle regulation, genome stability and pathogenicity in Proteobacteria , , we investigated the effects of mamA deletion under a number of different conditions. There were no distinguishable differences in growth between wildtype H37Rv, ΔmamA, and complemented strains in vitro under standard growth conditions (Figure 6A and data not shown). Sensitivity to reactive nitrogen and oxygen species was assessed and no significant differences in survival were observed among the strains (Figures S4A and S4B). ΔmamA and complemented strains did not differ in their abilities to compete with wildtype H37Rv in murine infections (Figure 6B and Figure S5). Mutation rates were likewise unaffected by deletion of mamA; this was unsurprising given that Mycobacteria lack homologs of the key proteins involved in methyl-directed DNA repair in Proteobacteria (Table S3) .
Figure 6. Deletion of mamA does not grossly affect growth rate or fitness of M. tuberculosis during mouse infection.
(A) The indicated H37Rv-derived strains were normalized at a calculated optical density of 0.01 in Sauton's media and monitored by optical density on the days indicated. Points indicate the mean of triplicate cultures and error bars denote standard deviation. Similar results were obtained in 7H9 medium and by plating for CFU. (B) Mice were infected by the aerosol route with approximately 10,000 CFU of a mixture of unmarked wildtype H37Rv and one of three isogenic mamA mutants marked with kanamycin resistance. Groups of four mice per condition were sacrificed at the indicated time points and the lung burden of total and marked bacilli was determined. The mean proportion of marked bacteria is indicated. Error bars denote standard deviation.doi:10.1371/journal.ppat.1003419.g006
Changes in gene expression are often associated with adaptation to new conditions, and we reasoned that MamA might be necessary for adapting to an environment that is not modeled accurately in the mouse. Humans develop hypoxic granulomas that are thought to slow or even arrest the growth of M. tuberculosis, but the lesions observed in mice are less organized and remain oxygenated –. Therefore, we therefore tested the ability of wildtype H37Rv, ΔmamA and complemented strains survive in hypoxic culture. Bacteria were seeded into vials with a defined headspace/liquid ratio, sealed and incubated with slow stirring, allowing the bacteria to gradually deplete the enclosed oxygen supply and enter a non-replicating state . While all strains displayed reduced viability over time as measured by colony forming units (CFU), the ΔmamA strain died at a significantly faster rate than the wildtype parent and the complemented strain (Figure 7A). To assess the viability of the bacteria exposed to hypoxia using a complementary method, a portion of each culture was removed and treated with fluorescein diacetate at day 28. Only viable cells have active intracellular esterases that convert fluorescein diacetate to fluorescein, inducing fluorescence . Consistent with the colony counts, hypoxic cultures of the wildtype and complemented strains had a high proportion of viable cells (~37–62%), while hypoxic cultures of the ΔmamA strain had significantly fewer viable cells (~23%) (Figure 7B–C).
Figure 7. MamA affects viability in hypoxic conditions.
The indicated strains of H37Rv were normalized to a calculated density of 3×106 CFU/ml and sealed in bottles containing equal volumes of culture and headspace. (A) Two bottles per strain were opened at the indicated timepoints and CFU/ml determined by plating. Error bars denote standard deviation. The negative slopes of the time points between day 14 and day 35 differ significantly between ΔmamA and the other two strains (P<0.05, linear regression of log10-transformed values according to the method in ). (B) After 28 days, samples of culture were treated with fluorescein diacetate and visualized by microscopy. Only live cells containing active intracellular esterases cleave fluorescein diacetate to produce fluorescent fluorescein. Scale bar = 10 µm. (C) Quantification of percent fluorescent bacteria in three-four fields at day 28. Error bars denote 95% confidence intervals. P<0.05 for all inter-strain comparisons (Fisher's exact test).doi:10.1371/journal.ppat.1003419.g007
In this work we demonstrate that Rv3263 encodes an adenine methyltransferase, MamA, which is responsible for all detectable DNA methylation in the Euro-American lineage strain of M. tuberculosis, H37Rv. By deleting mamA, we show that in M. tuberculosis, adenine methylation alters gene expression. Furthermore, mamA is required for optimal bacterial survival in a hypoxic environment. The expression changes mediated by MamA appear subtle in the bulk assays we used. One possible explanation is that methylation may direct greater expression differences in a subpopulation of cells. Single-cell methods will be required to explore this possibility and to determine whether DNA methylation allows heritable (epigenetic) regulation of gene expression in M. tuberculosis.
How does MamA alter gene expression? In each case that we have identified, the MamA site overlaps the sigma factor −10 binding site, a promoter region that is directly bound by the RNA polymerase holoenzyme during the initiation of transcription. Strikingly, the MamA sites are located at exactly the same position relative to the −10 sites in the four MamA-affected genes that we examined. This shared spatial configuration contrasts with the locus-specific relationships between Dam methylation sites and promoters at the known methylation-regulated genes in Proteobacteria , . The overlap between the promoter MamA sites and the sigma factor −10 binding sites is highly suggestive of a direct effect of methylation on expression of these genes. The lack of broad transcriptional changes or growth rate changes in the ΔmamA strain also suggests that the global physiology of the mutant is unperturbed under normal conditions, making indirect effects on transcription less likely. Indeed, the apparently restricted role of MamA under normal growth conditions may have allowed us to detect a category of subtle but direct effects on transcription that might also exist in E. coli but which are difficult to detect given the high frequency of Dam sites and dramatic effects of dam deletion on cellular physiology.
In the Proteobacteria, methylation has been shown to affect transcription by two broad mechanisms: (1), modulation of repressor binding, and (2), direct modulation of RNA polymerase's interactions with the promoter. Either of these mechanisms could underlie the MamA-dependent expression changes we observe and we propose several potential models for this effect. Methylation could potentially prevent binding of a transcriptional repressor, enhance promoter recognition by the RNA polymerase holoenzyme, increase the melting efficiency of the promoter, or enhance the stability of the open complex.
Three E. coli genes are known to be regulated by methylation sites that overlap their sigma factor −10 binding sites, as we observe here, but they do not share a common regulatory paradigm. One of these, dnaA, is regulated by repressor binding , ; another, IS10 transposase, is regulated by direct effects of methylation on RNA polymerase interaction with the promoter ; and the third, glnS, is Dam-regulated by unknown mechanisms . The dnaAp2 promoter harbors several Dam sites, one of which overlaps the −10 region. When the Dam sites are hemimethylated following DNA replication a repressor, SeqA, binds and inhibits transcription , , . Later in the cell cycle, when the promoter becomes fully methylated, expression resumes . This regulatory paradigm clearly falls into the category of methylation-state-dependent repressor binding, which includes other genes with Dam sites in different configurations, such as pap and agn43 , , , .
In the case of the IS10 transposase, methylation is thought to alter RNA polymerase interaction with the promoter. Here, methylation directly inhibits expression from the transposase promoter in vitro. Hemimethylated promoters have activity that is intermediate between fully methylated and unmethylated promoters . These findings suggest that methylation directly affects either the binding of the RNA polymerase holoenzyme to the promoter, open complex formation, or open complex stability. We note that the effect of methylation on IS10 expression is the opposite of what we observe for MamA-affected genes, and that the methylated bases lie at different positions within the −10 site in the two systems .
There are several mechanisms by which DNA methylation may affect open complex formation and stability. DNA is initially melted over a region extending from the second bp of the −10 site to just past the TSS in order to form open complexes . This process involves physical interaction between the −10 site and the 2.4 region of sigma as well as a more recently identified interaction between the DNA shortly downstream of the −10 site and the 1.2 region of sigma –. N6-MdA can reduce the melting temperature of DNA heteroduplexes in vitro , which may make open complex formation more thermodynamically favorable. DNA melting efficiency is thought to be important in open complex formation in part because of the AT-rich nature of −10 sites in most bacteria, and because a GC-rich region between the −10 site and TSS is important for stringent control of some promoters , , . In MamA-affected genes, N6-MdA in the −10 site and between the −10 site and TSS could therefore potentially enhance open complex formation and consequently increase expression.
It is also possible that region 1.2 of sigma could make direct contact with the N6-MdA located between the −10 site and TSS on the non-template strand, and that such an interaction could increase open complex stability directly. Region 1.2 of sigma was shown in E. coli to make direct contact with bases on the non-template strand between the −10 site and TSS of rrnB P1 and λPR , . In Bacillus subtilis, changing the base at position −5 of rrnB P1 from T to A resulted in a decrease in sensitivity of the promoter to GTP levels, indicating that the stability of its open complex was increased . Together, these data suggest that the sequence of the region between the −10 site and the TSS matters for reasons beyond GC content, and the non-template strand in particular plays an important role. In vitro studies will be required to elucidate the effects of MamA-mediated methylation on interactions between M. tuberculosis promoters and RNA polymerase holoenzyme.
The MamA-affected genes are not obviously related with respect to pathway or function, although several are involved in stress responses. Rv0102 is an essential gene predicted to encode an integral membrane protein of unknown function . It is not reported to undergo major transcriptional changes , although it may be modestly induced by oxidative stress . Rv0142 is predicted to encode a DNA glycosylase and is strongly induced in response to oxidative stress in a σH-dependent fashion –. It may also be induced by nitrosative stress . CorA encodes a predicted magnesium and cobalt transporter that may be modestly induced by thioridazine, proton gradient disrupters, and oxidative stress , , . We find that induction of Rv0142 and corA in response to oxidative stress appears to occur normally in a ΔmamA strain (data not shown), suggesting that MamA methylation affects the basal transcription of these genes but not the higher-level transcription that occurs during oxidative stress. WhiB7 is a transcriptional regulator that is induced by stationary phase, multiple antibiotics, heat shock, and iron starvation; disruption of whiB7 leads to increased antibiotic susceptibility , . Further work is needed to understand whether the hypoxia-survival defect in ΔmamA is related to the gene expression differences we detected or is a result of other effects of mamA deletion on hypoxia-specific gene expression. Gene expression profiling of 2-week-old hypoxic cultures suggested that a small number of genes are differentially expressed in the absence of mamA, similar to the extent of gene expression changes under standard in vitro growth conditions. These include some genes influenced by MamA in aerobic growth and some novel potential targets (data not shown). Further studies are required to validate these findings and understand their implications for M. tuberculosis survival under hypoxic conditions.
Interestingly, MamA is partially inactivated by a point mutation in most strains of the Beijing lineage of M. tuberculosis, while a second methyltransferase, HsdM, is active in the Beijing lineage and inactivated by a point mutation in most Euro-American strains. Non-synonymous methyltransferase mutations are found in other M. tuberculosis lineages as well (TB Database, ), although the effects of these mutations on methyltransferase activity are unknown. A few of the oldest strains of the Beijing and Euro-American lineages, as well as strains from the rim of the Indian Ocean, appear to encode intact copies of both MamA and HsdM. Further work will be needed to understand how the roles of DNA methylation may differ according to genetic background. It is possible that MamA is important for fitness of Euro-American strains during infection-associated hypoxia, but is unnecessary or even detrimental in modern Beijing lineage strains due to their altered hypoxia-response gene regulatory networks , . Loss of MamA function may affect other aspects of the Beijing lineage strains. The insertion sequence IS6110 has a MamA site that overlaps both the inverted repeat and the presumed promoter for the transposase gene. The number of IS6110 elements is higher in Beijing lineage strains than in other lineages, suggesting that transposition may be more frequent in Beijing lineage strains . IS6110 activity may be beneficial because it introduces genetic variability into a clonal species that lacks opportunities for horizontal gene transfer . In E. coli IS10 transposition is altered by Dam through both expression-dependent and –independent mechanisms . Although we did not detect changes in IS6110 transposase expression in strains lacking mamA (data not shown), future studies may indicate an effect of MamA on IS6110 transposition rates.
In this work we report the first investigation of the functional effects of DNA methylation in M. tuberculosis, as well as basic characterization of where and how DNA methylation occurs in this globally important bacterium. Methylation enhances expression of several genes that have methylation sites located in identical positions within their promoters, consistent with a shared regulatory paradigm. The activity of the methyltransferase MamA is required for normal survival of hypoxia, indicating that it is likely an important mediator of adaptation to this physiologically relevant stressor. Different methyltransferases predominate in different lineages of M. tuberculosis, suggesting that methylation-mediated regulatory pathways may contribute to lineage-specific characteristics.
Materials and Methods
Animal experiments were performed in strict accordance with the National Institutes of Health guidelines for housing and care of laboratory animals, with institutional regulations after protocol review, and with approval by the Harvard Medical Area Standing Committee on Animals. The animal protocol was approved by the Harvard University IACUC (protocol number 03000).
Strain construction and culture conditions
M. tuberculosis strains were grown in Middlebrook 7H9 or 7H10 media supplemented with 10% OADC (Oleic Albumin Dextrose Catalase, Becton Dickinson), glycerol, and 0.05% Tween 80 unless otherwise specified. H37Rv strains were derived from the ATCC lineage. Unmarked mamA (Rv3263) and hsdM deletion strains were constructed by a two-step process. Plasmids pSS002 and pSS004 were derived from pJM1  and contained 1 kb of the sequence upstream and downstream of hsdM and mamA, respectively, with 24–27 base pairs of coding sequence and a stop codon in between the two flanks. Plasmids were linearized with restriction enzymes cutting within one of the flanks before transformation into H37Rv. Integrants were selected with 50 µg/ml hygromycin. Counterselection with 7% sucrose was followed by PCR screening to identify isolates that subsequently underwent second crossovers resulting in loss of the plasmid and hsdM or mamA coding sequences. Complementation vectors were derived from pJEB402, which integrates as a single copy into the L5 attB site . The mamA coding sequence and 33 upstream bases (assumed to contain the RBS) were cloned behind the MOP promoter present in pJEB402, creating plasmid pSS030. We performed PCR with primers containing a central mutation in order to change nt 809 of the coding sequence from “A” to “C” creating pSS040. An equivalent strategy was used to insert the active site mutations N127D and Y130A into plasmids pSS075 and pSS077, respectively. For complementation with wildtype hsdM, the hsdM coding sequence and 23 upstream nt assumed to contain the RBS were cloned behind the MOP promoter in pJEB402 to produce plasmid pSS079.
Genomic DNA isolation and Southern blotting
Cultures were grown to an optical density of between 0.7 and 1.1 unless otherwise specified. Cell pellets were inactivated by chloroform-methanol (ratio 2:1), pelleted, resuspended in 0.1M Tris and 1 mM EDTA, pH 8–9, and lysed with lysozyme overnight at a final concentration of 100 µg/mL. Lysates were treated with 1% SDS and 100 µg/mL proteinase K (IBI Scientific) (final concentrations) for 3 hours at 50°C followed by phenol-chloroform extraction according to standard procedures, RNase (MO BIO) treatment (25 µg/mL for 1 hour at 37°C), and a second phenol-chloroform extraction. Two µg DNA was digested with PvuII (NEB) for Southern blotting. Blotting was performed according to standard protocols. DIG-labeled probe was made and detected with Roche DIG DNA Labeling and Detection Kit and Roche DIG Wash and Block Buffer Set according to the manufacturer's instructions.
Plasmid isolation and sequence trace comparison
Plasmids for sequence trace comparison were constructed by digesting pMV762  with BamHI and HindIII (NEB) and ligating in an annealed oligonucleotide duplex containing the sequence of interest (see Table S1 for oligonucleotide sequences). pSS012 contains the full 10 base pair sequence and other variants are listed in Table S1. pMV762 contains multiple complete and partial MamA sites, and these were sequenced as well. Plasmids were isolated from M. tuberculosis using a variation of a published protocol . Briefly, 30 ml of culture was pelleted and inactivated by overnight incubation with a 4:1 ratio of chloroform:methanol at 4°C. After centrifugation and removal of the liquid phases, pellets were resuspended in 200 µl of lysozyme solution  and incubated 4–18 h at 4°C. 400 µl of alkaline SDS solution  was added and samples incubated 30 min at 4°C with agitation. Buffer N3 (700 µl) from a Qiagen miniprep kit was added and samples centrifuged at maximum speed for 10 min. The supernatant was then applied to a Qiagen miniprep spin column and sample was processed according to the manufacturer's instructions. E. coli derived plasmids were propagated in both DH5-alpha and a dam dcm deletion strain (NEB). No differences were observed between sequence traces from the two E. coli strains.
Five µg of DNA was digested to nucleosides enzymatically with the addition of deaminases to reduce artifactual deamination due to contaminating deaminases in commercial enzyme preparations . Isotopically labeled internal standards for 5-MdC and N6-MdA were synthesized and spiked into the digestion reactions (EGP manuscript in preparation and ). An HPLC method that separates all four methylated products and the canonical nucleosides was developed utilizing a Cogent Diamond Hydride aqueous normal phase column (2.1×250 mm, 4 µm particle, 100 Å pore size; Microsolv Technology Corporation, Eatontown, NJ) with an isocratic step gradient of 0.1% acetic acid in acetonitrile/water (EGP manuscript in preparation). The LC-MS/MS analysis was performed on an Agilent 1100 HPLC coupled to an AB Sciex API 3000 triple quadrupole mass spectrometer in positive ion multiple reaction monitoring mode utilizing only 50–200 ng of DNA. Transitions monitored were m/z 266-150 (N6MdA), m/z 271-154 ([15N5]6-MdA), m/z 242-126 (5-MdC/N4MdC), m/z 254-133 ([13C915N3]-5-MdC), m/z 258-142 (5-hydroxymethylcytidine), m/z 252-136 (dA), m/z 243-127 (dT), m/z 228-112 (dC), and m/z 268-152 (dG). For each case, the monitored transition represents the loss of the 2′-deoxyribose. The areas under the curve of each nucleoside transition were quantitated and compared to calibration curves (r = 0.99). There were three biological replicates and at least two technical replicates per sample.
Female C57BL/6 mice were purchased from Jackson Laboratory (Bar Harbor, ME). Freshly grown cultures of wildtype H37Rv (kanamycin sensitive), ΔmamA::pJEB402, ΔmamA::pSS030, and ΔmamA::pSS040 (kanamycin resistant) were washed and cell densities were estimated by optical density. Equal quantities of wildtype bacteria were mixed with each of the three marked strains in order to perform three separate competition experiments. Mice were infected by the aerosol route with approximately 104 CFU. Four mice per group were sacrificed at the indicated time points and bacterial burden in the lung and spleen were determined by plating homogenized organs on plates both with and without 25 µg/ml kanamycin. Animal experiments were performed in accordance with the National Institutes of Health guidelines for housing and care of laboratory animals, with institutional regulations after protocol review, and with approval by the Harvard Medical Area Standing Committee on Animals.
RNA isolation and cDNA synthesis
RNA was isolated from cultures grown to OD 0.8–0.9 in the absence of antibiotics. Twenty ml of culture was added to 20 ml of RNAlater (Ambion) and incubated for 10 min. Ten ml of water was added immediately before centrifugation for 15 min. Pellets were resuspended in one ml Trizol (Invitrogen) and subject to bead-beating for 45 s and 30 s in a FastPrep-24 instrument (MP) before continuing according to the manufacturer's instructions. RNA samples were then treated with 10 U DNase Turbo (Ambion) for 1 h and purified with an RNeasy kit (Qiagen) according to the manufacturer's instructions, with the addition of RNaseOUT (Invitrogen) to the water used for elution. For quantitative PCR and TSS mapping, cDNA was synthesized as follows. One µg of RNA was mixed with 1.3 µl of 3 mg/ml random hexamers (Invitrogen), denatured at 70°C for 10 min and snap-cooled on ice before adding 4 µl 5X Superscript First Strand Buffer, 1 µl of dNTPs at 10 mM each, 0.4 µl of 500 mM DTT, 1 µl RNaseOUT, and 1 µl Superscript III (Invitrogen). Reactions were performed overnight at 42°C. RNA was degraded with the addition of 10 µl each 500 mM EDTA and 1 N NaOH and heating to 65°C for 15 min, followed by neutralization with 25 µl of 1M Tris pH 7.5. cDNA was then purified over Qiagen MinElute columns according to the manufacturer's instructions.
Quantitative PCR (qPCR)
qPCR primers are listed in Table S4. Each 20 µl reaction contained 100–200 pg of cDNA, 2.5 pg of each primer, and 10 µl of iTaq SYBR Green Supermix with ROX (Biorad). Reactions were run in an Applied Biosystems 7300 Real Time PCR System with the following program: 50°C/2 min, 95°C/5 min, and 40 cycles of 95°C/15 s and 61°C/30 s. Expression values normalized to sigA were calculated by the Δct method. Expression differences were compared by ANOVA with Tukey's post-test using GraphPad Prism 5. qPCR was performed on separate biological replicates from those used for microarray analysis.
RNA was extracted from triplicate cultures of indicated strains as described above for expression analysis with the Affymetrix custom-designed GeneChip MTbH37Rva520730F for M. tuberculosis (GEO platform number GPL17082, designed at the Broad Institute). Microarrays were run by the Boston University Microarray Core, who prepared the probes, hybridized and scanned the arrays according to the manufacturer's directions for prokaryotic samples with high GC content. Expression estimates were derived from probe-level hybridization intensities using RMA  in Expression Console (Affymetrix). Differential expression of non-intergenic features was assessed using 1-way ANOVA and for each ANOVA p-value we calculated a False Discovery Rate (FDR) using the method of Benjamini and Hochberg  to account for the large number of genomic features we interrogated. The ANOVA and FDR calculations were done using version 2.14 of the R Language for Statistical Computing . Data are available on GEO, accession number GSE46432.
Transcriptional start site (TSS) mapping
Total RNA samples were subject to two consecutive rounds of rRNA depletion with the MICROBExpress kit (Ambion) according to the manufacturer's instructions. To convert the natural 5′ triphosphates of mRNAs to 5′ monophosphates, approximately one µg of enriched mRNA was treated with 5′Polyphosphatase (Epicentre) for 30 minutes at 37°C in a 10 µl reaction containing 1 µl of enzyme and the supplied buffer, followed by RNeasy purification (Qiagen). The resulting sample was then circularized in 50 µl reactions contained 200 ng of RNA, 2 µl of T4 RNA ligase I (Epicentre), and ATP and buffer according to the manufacturer's recommendations in a final volume of 50 µl. Reactions were allowed to proceed for 2 h at 37°C and purified with RNeasy. cDNA was synthesized as described. Primer sets for genes of interest were designed such that the forward primer annealed approximately 100 base pairs upstream of the stop codon and the reverse primer annealed approximately 150–200 base pairs downstream of the start codon (Table S5). PCR reactions were in 25 µl volumes and contained 0.25 µl of Phusion polymerase (Finnymes), 1.25 pg of each primer, 0.2 µl of 25 mM each dNTPs, 1X GC buffer (Finnzymes), and 12–15 ng of cDNA. Reactions were performed with genomic DNA and with cDNA derived from non-circularized RNA for comparison. Cycler conditions were 98°C/2 min, 30 cycles of 98°C/15 s, 60°C/15 s, 72°C/15 s, and a final extension of 72°C/5 min. Entire reactions were then run on a 1% agarose gel. Bands present in reactions templated from circularized samples but absent in reactions templated from non-circularized samples or genomic DNA were excised and purified with Qiagen spin columns. One or two bands were identified for each gene, and some were sharp and distinct while others appeared as smears. Entire gel-extracted products were then concentrated under vacuum and subjected to a second round of PCR with the same primers, scaled up to 50 µl and with the addition of 1 µl of DMSO. Entire reactions were again run on a gel and the purified products were sequenced directly with their respective PCR primers.
Hypoxia survival experiments
The indicated strains of H37Rv were grown in 7H9 media supplemented with ADC and Tween-80 containing selective antibiotics if necessary. Seed cultures were washed twice, normalized and inoculated at a calculated density of 3×106 cfu/ml into 31 ml fresh media without antibiotics in a rubber stopper-sealed serum bottle (62 ml total volume). Cultures were shaken at ~120 rpm at 37°C with an intermittent manual homogenization in case of cell precipitation. Two bottles per strain were opened at the indicated time points and cfu/ml was determined after serial dilutions and plating onto 7H10 agar supplemented with OADC.
At 28 days, each hypoxic culture used for CFU determination was also was stained with fluorescein diacetate. 10 ml of culture was pelleted and resuspended in 2 ml of PBS with 0.05% Tween-80. Fluorescein diacetate was prepared as a 100X stock in acetonitrile and methanol (1:1) and added to resuspended cells to a final concentration of 50 µg/ml. After 30 minutes of incubation at 37°C, the dye-treated cells were washed with PBS-tween to remove the residual dye and then fixed with formalin. Fluorescent cells were visualized microscopically (DeltaVison, AppliedPrecision Inc.) using identical exposure settings for all strains. Death rates were calculated by linear regression analysis of log10-transformed data for time points between day 14 and day 35 (inclusive) in GraphPad Prism 5, which compares the significance of differences in slope using the method described in .
Expression of mamA in M. tuberculosis H37Rv. Expression of mamA relative to sigA was measured by qPCR. Expression of mamA was not detectable in the ΔmamA strain. The complemented strain displays approximately 3.5-fold more mamA expression than the wildtype strain, likely because the complementation vector contains a Mycobacterial optimized promoter (MOP) in place of the native promoter. Error bars denote standard deviation of mean of technical triplicates.
Quantification of sequence trace comparisons. The area under the curve (AUC) was determined for each peak in each of the sequence traces displayed in Figure 2A (Adobe Photoshop CS5) and normalized to the mean AUC for that trace. The percent difference in AUC in traces from plasmid isolated from M. tuberculosis strains compared to E. coli was determined for each peak. Gray shading indicates the mean percent difference plus two standard deviations; differences that exceeded this threshold were considered to be significant. (A) Quantification of “Top Strand” traces shown in Figure 2A. (B) Quantification of “Bottom Strand” traces shown in Figure 2A. Note that the peaks for nucleotides C4 and C5 overlapped substantially and were therefore analyzed together.
Confirmation of the whiB1 TSS. The TSS of whiB1 was mapped as in Figure 5. TSS is indicated by the black arrow.
Deletion of mamA does not affect sensitivity to nitrosative or oxidative stress in strain H37Rv. (A) Log-phase cultures were exposed to 10 mM DETA-NO for 24 and then plated for CFUs to assess survival compared to untreated cultures. Mean percent survival of triplicate cultures is shown. Error bars denote standard deviation. Differences between strains are not significant (t-test). Data are representative of two independent experiments. (B) Late log-phase cultures were pelleted and resuspended to OD 0.2 in catalase-free media with the addition of H2O2 to the indicated final concentrations. Growth over the next four days was monitored by OD. Mean ODs of triplicate cultures are shown. Error bars are omitted for the sake of clarity.
MamA status does not affect growth of H37Rv in mice. Mice were infected by the aerosol route with approximately 10,000 CFU of a mixture of unmarked wildtype H37Rv and one of three isogenic mamA mutants marked with kanamycin resistance. Groups of four mice per condition were sacrificed at the indicated time points and the lung burden of total and marked bacilli was determined. The mean CFU on 7H10 plates without drug (total M. tuberculosis), with kanamycin (mutant strain), and the calculated difference (wildtype H37Rv) are shown. Error bars denote standard deviation. (A) Infection with a mixture of unmarked H37Rv and kanR H37Rv ΔmamA. (B) Infection with a mixture of unmarked H37Rv and kanR H37Rv ΔmamA::mamA. (C) Infection with a mixture of unmarked H37Rv and kanR H37Rv ΔmamA::mamAE270A. Data are from the same experiment as shown in Figure 6B.
Structures of methylated 2′deoxynucleosides examined in this study. Figure made in ChemDraw.
Putative MamA recognition sequences tested by sequence trace comparison.
Complete aerobic microarray dataset.
Mutation rates in wildtype and ΔmamA strains.
Primers used for quantitative PCR.
Primers used for transcriptional start site mapping.
We thank Michael Chase for assistance with data analysis; Yuriy Alekseyev, Marc Lenburg, and Adam Gower at the Boston University Microarray Core and Brian Weiner at the Broad Institute for assistance with microarray experiments; Noman Siddiqi and Larry Pipkin at the Harvard School of Public Health BSL3 facility for assistance with animal husbandry and aerosol infections; Eric Rubin, Harvard School of Public Health, and Martin Marinus, University of Massachusetts Medical School, for advice; and Joseph Campbell at the National Institutes of Health for critical reading of the manuscript. Mass spectrometry studies were performed in the Bioanalytical Facilities Core of the MIT Center for Environmental Health Sciences.
Conceived and designed the experiments: SSS EGP SHB CMS PCD SMF. Performed the experiments: SSS EGP SHB RRS. Analyzed the data: SSS EGP SHB RRS CMS PCD SMF. Wrote the paper: SSS SMF.
- 1. World Health Organization (2011) Global Tuberculosis Control 2011. World Health Organization.
- 2. Militello KT, Simon RD, Qureshi M, Maines R, VanHorne ML, et al. (2012) Conservation of Dcm-mediated cytosine DNA methylation in Escherichia coli. FEMS Microbiology Letters 328: 78–85 doi:10.1111/j.1574-6968.2011.02482.x.
- 3. Kahramanoglou C, Prieto AI, Khedkar S, Haase B, Gupta A, et al. (2012) Genomics of DNA cytosine methylation in Escherichia coli reveals its role in stationary phase transcription. Nat Commun 3: 886 doi:10.1038/ncomms1878.
- 4. Suzuki MM, Bird A (2008) DNA methylation landscapes: provocative insights from epigenomics. Nature Reviews Genetics 9: 465–476 doi:10.1038/nrg2341.
- 5. Casadesus J, Low D (2006) Epigenetic Gene Regulation in the Bacterial World. Microbiology and Molecular Biology Reviews 70: 830–856 doi:10.1128/MMBR.00016-06.
- 6. Lu M, Campbell JL, Boye E, Kleckner N (1994) SeqA: a negative modulator of replication initiation in E. coli. Cell 77: 413–426. doi: 10.1016/0092-8674(94)90156-2
- 7. Braaten BA, Nou X, Kaltenbach LS, Low DA (1994) Methylation patterns in pap regulatory DNA control pyelonephritis-associated pili phase variation in E. coli. Cell 76: 577–588. doi: 10.1016/0092-8674(94)90120-1
- 8. Campbell JL, Kleckner N (1990) E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork. Cell 62: 967–979. doi: 10.1016/0092-8674(90)90271-f
- 9. Blyn LB, Braaten BA, Low DA (1990) Regulation of pap pilin phase variation by a mechanism involving differential dam methylation states. EMBO J 9: 4045–4054.
- 10. Broadbent SE, Balbontin R, Casadesus J, Marinus MG, van der Woude M (2007) YhdJ, a Nonessential CcrM-Like DNA Methyltransferase of Escherichia coli and Salmonella enterica. Journal of Bacteriology 189: 4325–4327 doi:10.1128/JB.01854-06.
- 11. Braun RE, Wright A (1986) DNA methylation differentially enhances the expression of one of the two E. coli dnaA promoters in vivo and in vitro. Mol Gen Genet 202: 246–250. doi: 10.1007/bf00331644
- 12. Kedar GC, Ozcan F, Guzmán EC, Smith DW, Newman VG, et al. (2000) Role of DNA methylation at GATC sites in the dnaA promoter, dnaAp2. J Mol Microbiol Biotechnol 2: 301–310.
- 13. Palmer BR, Marinus MG (1994) The dam and dcm strains of Escherichia coli–a review. Gene 143: 1–12. doi: 10.1016/0378-1119(94)90597-5
- 14. Iyer RR, Pluciennik A, Burdett V, Modrich PL (2006) DNA Mismatch Repair: Functions and Mechanisms. Chem Rev 106: 302–323 doi:10.1021/cr0404794.
- 15. Henderson IR, Owen P (1999) The Major Phase-Variable Outer Membrane Protein of Escherichia coli Structurally Resembles the Immunoglobulin A1 Protease Class of Exported Protein and Is Regulated by a Novel Mechanism Involving Dam and OxyR. Journal of Bacteriology 181: 2132–2141.
- 16. Roberts D, Hoopes B, McClure W, Kleckner N (1985) IS10 transposition is regulated by DNA adenine methylation. Cell 43: 117–130. doi: 10.1016/0092-8674(85)90017-0
- 17. Wallecha A, Munster V, Correnti J, Chan T, van der Woude M (2002) Dam-and OxyR-dependent phase variation of agn43: essential elements and evidence for a new role of DNA methylation. Journal of Bacteriology 184: 3338. doi: 10.1128/jb.184.12.3338-3347.2002
- 18. Lobner-Olesen A, Hansen FG, Rasmussen KV, Martin B, Kuempel PL (1994) The initiation cascade for chromosome replication in wild-type and Dam methyltransferase deficient Escherichia coli cells. EMBO J 13: 1856–1862.
- 19. Bale A, d'Alarcao M, Marinus MG (1979) Characterization of DNA adenine methylation mutants of Escherichia coli K12. Mutat Res 59: 157–165. doi: 10.1016/0027-5107(79)90153-2
- 20. Marinus MG, Poteete A, Arraj JA (1984) Correlation of DNA adenine methylase activity with spontaneous mutability in Escherichia coli K-12. Gene 28: 123–125. doi: 10.1016/0378-1119(84)90095-7
- 21. Oshima T, Wada C, Kawagoe Y, Ara T, Maeda M, et al. (2002) Genome-wide analysis of deoxyadenosine methyltransferase-mediated control of gene expression in Escherichia coli. Molecular Microbiology 45: 673–695. doi: 10.1046/j.1365-2958.2002.03037.x
- 22. Robbins-Manke JL, Zdraveski ZZ, Marinus M, Essigmann JM (2005) Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase-and mismatch repair-deficient Escherichia coli. Journal of Bacteriology 187: 7027. doi: 10.1128/jb.187.20.7027-7037.2005
- 23. Lobner-Olesen A, Marinus MG, Hansen FG (2003) Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis. Proc Natl Acad Sci USA 100: 4672. doi: 10.1073/pnas.0538053100
- 24. Brunet YR, Bernard CS, Gavioli M, Lloubès R, Cascales E (2011) An Epigenetic Switch Involving Overlapping Fur and DNA Methylation Optimizes Expression of a Type VI Secretion Gene Cluster. PLoS Genet 7: e1002205 doi:10.1371/journal.pgen.1002205.g007.
- 25. Gammie AE, Crosa JH (1991) Roles of DNA adenine methylation in controlling replication of the REPI replicon of plasmid pColV-K30. Molecular Microbiology 5: 495–503. doi: 10.1111/j.1365-2958.1991.tb02133.x
- 26. Haagmans W, van der Woude M (2000) Phase variation of Ag43 in Escherichia coli: Dam-dependent methylation abrogates OxyR binding and OxyR-mediated repression of transcription. Molecular Microbiology 35: 877–887. doi: 10.1046/j.1365-2958.2000.01762.x
- 27. Hale WB, Van Der Woude MW, Braaten BA, Low DA (1998) Regulation of uropathogenic Escherichia coli adhesin expression by DNA methylation. Mol Genet Metab 65: 191–196 doi:10.1006/mgme.1998.2744.
- 28. Marinus MG (1985) DNA methylation influences trpR promoter activity in Escherichia coli K-12. Mol Gen Genet 200: 185–186. doi: 10.1007/bf00383334
- 29. Broadbent SE, Davies MR, Van Der Woude MW (2010) Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism. Molecular Microbiology 77: 337–353 doi:10.1111/j.1365-2958.2010.07203.x.
- 30. Camacho EM, Casadesus J (2005) Regulation of traJ transcription in the Salmonella virulence plasmid by strand-specific DNA adenine hemimethylation. Molecular Microbiology 57: 1700–1718 doi:10.1111/j.1365-2958.2005.04788.x.
- 31. Srivastava R, Gopinathan KP, Ramakrishnan T (1981) Deoxyribonucleic acid methylation in mycobacteria. Journal of Bacteriology 148: 716–719.
- 32. Hemavathy KC, Nagaraja V (1995) DNA methylation in mycobacteria: absence of methylation at GATC (Dam) and CCA/TGG (Dcm) sequences. FEMS Immunol Med Microbiol 11: 291–296. doi: 10.1111/j.1574-695x.1995.tb00159.x
- 33. van Soolingen D, de Haas PE, Blumenthal RM, Kremer K, Sluijter M, et al. (1996) Host-mediated modification of PvuII restriction in Mycobacterium tuberculosis. Journal of Bacteriology 178: 78–84.
- 34. van der Spuy GD, Kremer K, Ndabambi SL, Beyers N, Dunbar R, et al. (2009) Changing Mycobacterium tuberculosis population highlights clade-specific pathogenic characteristics. Tuberculosis (Edinb) 89: 120–125 doi:10.1016/j.tube.2008.09.003.
- 35. Parwati I, van Crevel R, van Soolingen D (2010) Possible underlying mechanisms for successful emergence of the Mycobacterium tuberculosis Beijing genotype strains. The Lancet Infectious Diseases 10: 103–111 doi:10.1016/S1473-3099(09)70330-5.
- 36. European Concerted Action on New Generation Genetic Markers and Techniques for the Epidemiology and Control of Tuberculosis (2006) Beijing/W genotype Mycobacterium tuberculosis and drug resistance. Emerg Infect Dis 12: 736–743. doi: 10.3201/eid1205.050400
- 37. Homolka S, Niemann S, Russell DG, Rohde KH (2010) Functional Genetic Diversity among Mycobacterium tuberculosis Complex Clinical Isolates: Delineation of Conserved Core and Lineage-Specific Transcriptomes during Intracellular Survival. PLoS Pathog 6: e1000988 doi:10.1371/journal.ppat.1000988.t001.
- 38. Reed MB, Domenech P, Manca C, Su H, Barczak AK, et al. (2004) A glycolipid of hypervirulent tuberculosis strains that inhibits the innate immune response. Nature 431: 84–87 doi:10.1038/nature02837.
- 39. Reed MB, Gagneux S, DeRiemer K, Small PM, Barry CE (2007) The W-Beijing Lineage of Mycobacterium tuberculosis Overproduces Triglycerides and Has the DosR Dormancy Regulon Constitutively Upregulated. Journal of Bacteriology 189: 2583–2589 doi:10.1128/JB.01670-06.
- 40. Fallow A, Domenech P, Reed MB (2010) Strains of the East Asian (W/Beijing) Lineage of Mycobacterium tuberculosis Are DosS/DosT-DosR Two-Component Regulatory System Natural Mutants. Journal of Bacteriology 192: 2228–2238 doi:10.1128/JB.01597-09.
- 41. Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, et al. (2003) A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Research 31: 1805–1812. doi: 10.1093/nar/gkg274
- 42. Roberts RJ, Vincze T, Posfai J, Macelis D (2010) REBASE—a database for DNA restriction and modification: enzymes, genes and genomes. Nucleic Acids Research 38: D234–D236. doi: 10.1093/nar/gkp874
- 43. Reddy TBK, Riley R, Wymore F, Montgomery P, DeCaprio D, et al. (2009) TB database: an integrated platform for tuberculosis research. Nucleic Acids Research 37: D499–D508 doi:10.1093/nar/gkn652.
- 44. Bart A, Van Passel M, Van Amsterdam K, Van Der Ende A (2005) Direct detection of methylation in genomic DNA. Nucleic Acids Research 33: e124–e124. doi: 10.1093/nar/gni121
- 45. Rao BS, Buckler-White A (1998) Direct visualization of site-specific and strand-specific DNA methylation patterns in automated DNA sequencing data. Nucleic Acids Research 26: 2505–2507. doi: 10.1093/nar/26.10.2505
- 46. Bart A, Pannekoek Y, Dankert J, Van Der Ende A (2001) NmeSI Restriction-Modification System Identified by Representational Difference Analysis of a Hypervirulent Neisseria meningitidis Strain. Infection and Immunity 69: 1816–1820. doi: 10.1128/iai.69.3.1816-1820.2001
- 47. de Miranda AB, Alvarez-Valin F, Jabbari K, Degrave WM, Bernardi G (2000) Gene expression, amino acid conservation, and hydrophobicity are the main factors shaping codon preferences in Mycobacterium tuberculosis and Mycobacterium leprae. Journal of molecular evolution 50: 45–55 doi:10.1007/s002399910006.
- 48. Wilson GG (1992) Amino acid sequence arrangements of DNA-methyltransferases. Meth Enzymol 216: 259–279. doi: 10.1016/0076-6879(92)16026-g
- 49. Willcock DF, Dryden DT, Murray NE (1994) A mutational analysis of the two motifs common to adenine methyltransferases. EMBO J 13: 3902–3908.
- 50. Pues H, Bleimling N, Holz B, Wölcke J, Weinhold E (1999) Functional roles of the conserved aromatic amino acid residues at position 108 (motif IV) and position 196 (motif VIII) in base flipping and catalysis by the N6-adenine DNA methyltransferase from Thermus aquaticus. Biochemistry 38: 1426–1434 doi:10.1021/bi9818016.
- 51. Guyot JB, Grassi J, Hahn U, Guschlbauer W (1993) The role of the preserved sequences of Dam methylase. Nucleic Acids Research 21: 3183–3190. doi: 10.1093/nar/21.14.3183
- 52. Roth M, Helm-Kruse S, Friedrich T, Jeltsch A (1998) Functional roles of conserved amino acid residues in DNA methyltransferases investigated by site-directed mutagenesis of the EcoRV adenine-N6-methyltransferase. J Biol Chem 273: 17333–17342. doi: 10.1074/jbc.273.28.17333
- 53. Bheemanaik S, Reddy YVR, Rao DN (2006) Structure, function and mechanism of exocyclic DNA methyltransferases. Biochem J 399: 177–190 doi:10.1042/BJ20060854.
- 54. Kong H, Smith CL (1997) Substrate DNA and cofactor regulate the activities of a multi-functional restriction-modification enzyme, BcgI. Nucleic Acids Research 25: 3687–3692. doi: 10.1093/nar/25.18.3687
- 55. Cheng X (1995) Structure and function of DNA methyltransferases. Annual review of biophysics and biomolecular structure 24: 293–318. doi: 10.1146/annurev.biophys.24.1.293
- 56. Agarwal N, Raghunand TR, Bishai WR (2006) Regulation of the expression of whiB1 in Mycobacterium tuberculosis: role of cAMP receptor protein. Microbiology 152: 2749–2756 doi:10.1099/mic.0.28924-0.
- 57. Giacomodonato MN, Sarnacki SH, Llana MN, Cerquetti MC (2009) Dam and its role in pathogenicity of Salmonella enterica. J Infect Dev Ctries 3: 484–490. doi: 10.3855/jidc.465
- 58. López-Garrido J, Casadesus J (2012) Crosstalk between virulence loci: regulation of Salmonella enterica pathogenicity island 1 (SPI-1) by products of the std fimbrial operon. PLoS ONE 7: e30499 doi:10.1371/journal.pone.0030499.
- 59. Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, et al. (1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393: 537–544 doi:10.1038/31159.
- 60. Via LE, Lin PL, Ray SM, Carrillo J, Allen SS, et al. (2008) Tuberculous Granulomas Are Hypoxic in Guinea Pigs, Rabbits, and Nonhuman Primates. Infection and Immunity 76: 2333–2340 doi:10.1128/IAI.01515-07.
- 61. Tsai MC, Chakravarty S, Zhu G, Xu J, Tanaka K, et al. (2006) Characterization of the tuberculous granuloma in murine and human lungs: cellular composition and relative tissue oxygen tension. Cell Microbiol 8: 218–232 doi:10.1111/j.1462-5822.2005.00612.x.
- 62. Aly S, Wagner K, Keller C, Malm S, Malzan A, et al. (2006) Oxygen status of lung granulomas in Mycobacterium tuberculosis-infected mice. J Pathol 210: 298–305 doi:10.1002/path.2055.
- 63. Wayne LG, Hayes LG (1996) An in vitro model for sequential study of shiftdown of Mycobacterium tuberculosis through two stages of nonreplicating persistence. Infection and Immunity 64: 2062–2069.
- 64. Jarnagin JL, Luchsinger DW (1980) The use of fluorescein diacetate and ethidium bromide as a stain for evaluating viability of mycobacteria. Stain Technol 55: 253–258. doi: 10.3109/10520298009067249
- 65. Wion D, Casadesus J (2006) N6-methyl-adenine: an epigenetic signal for DNA–protein interactions. Nat Rev Micro 4: 183–192 doi:10.1038/nrmicro1350.
- 66. Plumbridge J, Söll D (1987) The effect of dam methylation on the expression of glnS in E. coli. Biochimie 69: 539–541. doi: 10.1016/0300-9084(87)90091-5
- 67. deHaseth PL, Zupancic ML, Record MT (1998) RNA polymerase-promoter interactions: the comings and goings of RNA polymerase. Journal of Bacteriology 180: 3019–3025.
- 68. Sachdeva P, Misra R, Tyagi AK, Singh Y (2010) The sigma factors of Mycobacterium tuberculosis: regulation of the regulators. FEBS Journal 277: 605–626 doi:10.1111/j.1742-4658.2009.07479.x.
- 69. Haugen SP, Ross W, Gourse RL (2008) Advances in bacterial promoter recognition and its control by factors that do not bind DNA. Nat Rev Micro 6: 507–519 doi:10.1038/nrmicro1912.
- 70. Haugen SP, Berkmen MB, Ross W, Gaal T, Ward C, et al. (2006) rRNA promoter regulation by nonoptimal binding of sigma region 1.2: an additional recognition element for RNA polymerase. Cell 125: 1069–1082 doi:10.1016/j.cell.2006.04.034.
- 71. Feklistov A, Darst SA (2011) Structural basis for promoter-10 element recognition by the bacterial RNA polymerase σ subunit. Cell 147: 1257–1269 doi:10.1016/j.cell.2011.10.041.
- 72. Engel JD, Hippel von PH (1978) Effects of methylation on the stability of nucleic acid conformations. Studies at the polymer level. Journal of Biological Chemistry 253: 927–934. doi: 10.1021/bi00717a013
- 73. Jung YH, Lee Y (1997) Escherichia coli rnpB promoter mutants altered in stringent response. Biochemical and Biophysical Research Communications 230: 582–586 doi:10.1006/bbrc.1996.6005.
- 74. Lamond AI, Travers AA (1985) Stringent control of bacterial transcription. Cell 41: 6–8. doi: 10.1016/0092-8674(85)90050-9
- 75. Haugen SP, Ross W, Manrique M, Gourse RL (2008) Fine structure of the promoter-sigma region 1.2 interaction. Proc Natl Acad Sci USA 105: 3292–3297 doi:10.1073/pnas.0709513105.
- 76. Sojka L, Kouba T, Barvík I, Sanderová H, Maderová Z, et al. (2011) Rapid changes in gene expression: DNA determinants of promoter regulation by the concentration of the transcription initiating NTP in Bacillus subtilis. Nucleic Acids Research 39: 4598–4611 doi:10.1093/nar/gkr032.
- 77. Sassetti CM, Boyd DH, Rubin EJ (2003) Genes required for mycobacterial growth defined by high density mutagenesis. Molecular Microbiology 48: 77–84. doi: 10.1046/j.1365-2958.2003.03425.x
- 78. Boshoff HIM (2004) The Transcriptional Responses of Mycobacterium tuberculosis to Inhibitors of Metabolism: NOVEL INSIGHTS INTO DRUG MECHANISMS OF ACTION. J Biol Chem 279: 40174–40184 doi:10.1074/jbc.M406796200.
- 79. Voskuil MI, Bartek IL, Visconti K, Schoolnik GK (2011) The response of mycobacterium tuberculosis to reactive oxygen and nitrogen species. Front Microbiol 2: 105 doi:10.3389/fmicb.2011.00105.
- 80. Fontan PA, Voskuil MI, Gomez M, Tan D, Pardini M, et al. (2009) The Mycobacterium tuberculosis Sigma Factor B Is Required for Full Response to Cell Envelope Stress and Hypoxia In Vitro, but It Is Dispensable for In Vivo Growth. Journal of Bacteriology 191: 5628–5633 doi:10.1128/JB.00510-09.
- 81. Manganelli R, Voskuil MI, Schoolnik GK, Dubnau E, Gomez M, et al. (2002) Role of the extracytoplasmic-function sigma factor sigma(H) in Mycobacterium tuberculosis global gene expression. Molecular Microbiology 45: 365–374. doi: 10.1046/j.1365-2958.2002.03005.x
- 82. Dutta NK, Mehra S, Kaushal D (2010) A Mycobacterium tuberculosis Sigma Factor Network Responds to Cell-Envelope Damage by the Promising Anti-Mycobacterial Thioridazine. PLoS ONE 5: e10069 doi:10.1371/journal.pone.0010069.t001.
- 83. Morris RP, Nguyen L, Gatfield J, Visconti K, Nguyen K, et al. (2005) Ancestral antibiotic resistance in Mycobacterium tuberculosis. Proc Natl Acad Sci USA 102: 12200–12205 doi:10.1073/pnas.0505446102.
- 84. Geiman DE, Raghunand TR, Agarwal N, Bishai WR (2006) Differential Gene Expression in Response to Exposure to Antimycobacterial Agents and Other Stress Conditions among Seven Mycobacterium tuberculosis whiB-Like Genes. Antimicrobial Agents and Chemotherapy 50: 2836–2841 doi:10.1128/AAC.00295-06.
- 85. Hanekom M, van der Spuy GD, Streicher E, Ndabambi SL, McEvoy CRE, et al. (2007) A Recently Evolved Sublineage of the Mycobacterium tuberculosis Beijing Strain Family Is Associated with an Increased Ability to Spread and Cause Disease. Journal of Clinical Microbiology 45: 1483–1490 doi:10.1128/JCM.02191-06.
- 86. McEvoy CRE, Falmer AA, van Pittius NCG, Victor TC, van Helden PD, et al. (2007) The role of IS6110 in the evolution of Mycobacterium tuberculosis. Tuberculosis 87: 393–404 doi:10.1016/j.tube.2007.05.010.
- 87. Farrow MF, Rubin EJ (2008) Function of a mycobacterial major facilitator superfamily pump requires a membrane-associated lipoprotein. Journal of Bacteriology 190: 1783–1791 doi:10.1128/JB.01046-07.
- 88. Lee MH, Pascopella L, Jacobs WR, Hatfull GF (1991) Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guérin. Proc Natl Acad Sci USA 88: 3111–3115. doi: 10.1073/pnas.88.8.3111
- 89. Steyn AJC, Joseph J, Bloom BR (2003) Interaction of the sensor module of Mycobacterium tuberculosis H37Rv KdpD with members of the Lpr family. Molecular Microbiology 47: 1075–1089. doi: 10.1046/j.1365-2958.2003.03356.x
- 90. Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7: 1513–1523. doi: 10.1093/nar/7.6.1513
- 91. Taghizadeh K, McFaline JL, Pang B, Sullivan M, Dong M, et al. (2008) Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry. Nat Protoc 3: 1287–1298 doi:10.1038/nprot.2008.119.
- 92. Divakar KJ, Reese CB (1982) 4-(1,2,4-Triazol-1-yl)- and 4-(3-nitro-1,2,4-triazol-1-yl)-1-(?-D-2,3,5-tri-O-acetylarabinofuranosyl)pyrimidin-2(1H)-ones.Valuable intermediates in the synthesis of derivatives of 1-(?-D-arabinofuranosyl)cytosine (ara-C). J Chem Soc, Perkin Trans 1: 1171–1176 doi:10.1039/p19820001171.
- 93. Irizarry RA, Bolstad BM, Collin F, Cope LM, Hobbs B, et al. (2003) Summaries of Affymetrix GeneChip probe level data. Nucleic Acids Research 31: e15. doi: 10.1093/nar/gng015
- 94. Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society Series B (Methodological) 289–300.
- 95. Team RDC (2010) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing Vienna Austria.
- 96. Zar JH (1984) Biostatistical Analysis Second Edition. 2nd edition. Prentice Hall. 1 pp.